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Dynasore
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Dynasore图片
CAS NO:304448-55-3
包装与价格:
包装价格(元)
5 mg电议
10 mg电议
25 mg电议
50 mg电议
100 mg电议
200 mg电议
500 mg电议
1 mL*10 mM(in DMSO)电议

产品名称
Dynamin Inhibitor I
产品介绍
Dynasore 是一种可渗透细胞的化学物质,可逆且非竞争性地抑制 Dynamin 1/2 的 GTPase 活性。 它还抑制线粒体动力蛋白 Drp1,对其他小 GTPase 没有影响。

产品描述

Dynasore, a cell-permeable chemical, can reversibly and non-competitivity inhibit GTPase activity of dynamin 1/2 (IC50: 15 μM in a cell-free assay). It also inhibits the mitochondrial dynamin Drp1, with no effect against other small GTPase.

体内活性

Dynasore ameliorates the motor dysfunction greatly at 3, 7, and 10 days after SCI in rats. Dynasore markedly enhances motor function which may be through inhibiting the activation of neuronal mitochondrial apoptotic pathway and astrocytic proliferation in rats after SCI[4].

激酶实验

RIP1 kinase assay: Phosphorylation of RIP1 requires its kinase activity. Expression constructs of FLAGtagged wild-type (WT) or a kinase-inactive point mutant of RIP1 (K45M) are are transfected into 293T cells and RIP1 kinase assay is performed as described in the Methods in the presence of [γ-32P]ATP for 30 min at 30℃. Samples are subjected to SDS-PAGE and RIP1 band is visualized by autoradiography. Relative intensities of radioactive bands are quantified and are shown (ratio) in this and all other autoradiographs. In parallel to kinase reactions, a sample of beads is subjected to western blot analysis using anti-RIP1 antibody to ensure equal protein amounts in kinase reactions.

细胞实验

Mouse ventricular myocytes are isolated from male adult C6/Black mouse. Cardiomyocytes subjects to 2 hours of drug treatment followed by oxidative stress (30 μM Water2 for 35 min). For ATP supplement experiments, the cells are treated with 3 mM ATP for 30 min before exposure to Water2. Cardiomyocyte survival and viability are analyzed by trypan blue exclusion (TBE) assay[3].

Cas No.

304448-55-3

分子式

C18H14N2O4

分子量

322.32

别名

Dynamin Inhibitor I

储存和溶解度

DMSO:32.2 mg/mL (100 mM)
Ethanol:1.6 mg/mL (5 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years