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CeMMEC1
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
CeMMEC1图片
CAS NO:440662-09-9
包装与价格:
包装价格(元)
5 mg电议
10 mg电议
25 mg电议
50 mg电议
1 mL*10 mM(in DMSO)电议

产品名称
CeMMEC1 HCl
产品介绍
CeMMEC1 是一种 BRD4 抑制剂,对 TAF1 有很好的亲和力,IC50为0.9 μM。

产品描述

CeMMEC1 is an inhibitor of BRD4, and also has a great affinity for TAF1(IC50=0.9 μM).

体外活性

CeMMEC1 exhibits high affinity activity for the bromodomains of CREBBP, EP300, BRD9 and the second bromodomain of TAF1.

激酶实验

TAF1 binding assays are conducted using the EPIgeneous Binding Domain kit B. Binding is determined by the displacement of an acetylated biotin peptide from a GST-tagged TAF1 protein using HTRF with a Eu3+-conjugated GST antibody donor and streptavidin-conjugated acceptor. Compounds (CeMMEC1) are dispensed into assay plates, ProxiPlate-384 Plus using an Echo 525 Liquid Handler. Binding assays are conducted in a final volume of 20 μL with 5 nM TAF1-GST, 50 nM peptide (SGRGK (ac)GGK (ac)GLGK (ac)GGAK (ac)RHRK (biotin)-acid), 6.25 nM Streptavidin-XL665, 1:200 Anti-GST-Eu3+ cryptate and 0.1% DMSO. Assay reagents are dispensed into plates using a Multidrop combi and incubated at room temperature for 3 h. Fluorescence is measured using a PHERAstar microplate reader using the HTRF module with dual emission protocol (A = excitation of 320 nm, emmission of 665 nm, and B = excitation of 320 nm, emission of 620 nm). Raw data are processed to give an HTRF ratio (channel A/B × 10,000), which is used to generate IC50 curves

细胞实验

REDS3 cells were treated with CeMMEC1(10 μM) incubating for 24 hours.

Cas No.

440662-09-9

分子式

C19H16N2O4

分子量

336.347

别名

CeMMEC1 HCl

储存和溶解度

DMSO:21 mg/mL(62.4 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years