您好,欢迎来到化工原料网! [登录] [免费注册]
化工原料网
位置:首页 > 产品库 > Gramine
立即咨询
咨询类型:
     
*姓名:
*电话:
*单位:
Email:
*留言内容:
请详细说明您的需求。
*验证码:
 
Gramine
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Gramine图片
CAS NO:87-52-5
包装与价格:
包装价格(元)
50 mg电议
1 mL*10 mM(in DMSO)电议

产品名称
芦竹碱
NSC 16892
Donaxine
产品介绍
Gramine是从芦竹中得到的一种生物碱,是脂联素受体激动剂,对 AdipoR2 和 AdipoR1 的IC50值分别为 3.2 和 4.2 μM。它也是小鼠和人β2-肾上腺素受体激动剂,具有抗肿瘤、抗炎和抗病毒作用。

产品描述

Gramine, a natural indole alkaloid, present in several plant species, acts as an active adiponectin receptor (AdipoR) agonist, with IC50s of 3.2 and 4.2 μM for AdipoR2 and AdipoR1, respectively. Gramine is also a human and mouse β2-Adrenergic receptor (β2-AR) agonist. Gramine has anti-tumor, anti-viral and anti-inflammatory properties.

激酶实验

Assay of protein kinase C: Protein kinase C is arrayed by measuring 32PI transferred from [gamma-32PI] ATP to lysine-rich histone type Ill-s. The reaction mixture (80 μL) contained 50 mM Tris-HCI. pH 7.4, 100 μM CaCl2, 10 mM MgCI2, 37.5 μL/mL histone type Ill-s, 10 μM [gamma-32PI] ATP (1250 cpm/pmol), 31 μM bovine brain phosphatidylserine and 0.5 μM 1,2 sn-dioleylglycerol. Fifteen μL of purified PKC (final concentration in assay 0.38 μg/mL) is added to the incubation mixture. After 10 min at 30°C, the reaction is stopped by addition of 30 μL of casein 30 mg/mL and 0.9 ml of 12% trichloroacetic acid. The acid precipitable material is collected by centrifugation, dissolved in 1N NaOH (100μL) and precipitated again with 1 ml of 12% trichloroacetic acid. The pellet is dissolved in 1N NaOH (100μL) and 32P incorporation is measured by scintillation counting in Aquasol.

Cas No.

87-52-5

分子式

C11H14N2

分子量

174.247

别名

芦竹碱;NSC 16892;Donaxine

储存和溶解度

H2O:<1 mgml
Ethanol:33 mg/mL (189.4 mM)
DMSO:33 mg/mL (189.4 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years