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7-Amino-4-methyl-3-coumarinylacetic Acid
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
7-Amino-4-methyl-3-coumarinylacetic Acid图片
CAS NO:106562-32-7
包装与价格:
包装价格(元)
25mg电议
50mg电议
100mg电议

产品介绍
7-Amino-4-methyl-3-coumarinyl acetate Acid 是一种荧光蛋白标记剂。
Cas No.106562-32-7
别名7-氨基-4-甲基-3-香豆素醋酸
Canonical SMILESNC1=CC=C2C(OC(C(CC(O)=O)=C2C)=O)=C1
分子式C12H11NO4
分子量233.2
溶解度DMF: 2mg/mL,DMSO: 3mg/mL,DMSO:PBS (pH 7.2) (1:6): 0.14mg/mL
储存条件Store at -20°C
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping ConditionEvaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
产品描述

7-Amino-4-methyl-3-coumarinylacetic acid (AMCA) is fluorescent protein labeling agent.1 It contains an N-hydroxysuccinimide ester that reacts with lysine residues to form photostable amide links. Upon activation with UV light, AMCA displays emission maxima of 400-460 nm. It has commonly been used in multiplex immunophenotyping applications.2,3,4

|1. Khalfan, H., Abuknesha, R., Rand-Weaver, M., et al. Aminomethyl coumarin acetic acid: A new fluorescent labelling agent for proteins. Histochem. J. 18(9), 497-499 (1986).|2. Ferri, G.-L., Gaudio, R.M., Castello, I.F., et al. Quadruple immunofluorescence: A direct visualization method. J. Histochem. Cytochem. 45(2), 155-158 (1997).|3. Nederlof, P.M., Robinson, D., Abuknesha, R., et al. Three-color fluorescence in situ hybridization for the simultaneous detection of multiple nucleic acid sequences. Cytometry 10(1), 20-27 (1989).|4. Gothot, A., Grosdent, J.-C., and Paulus, J.-M. A strategy for multiple immunophenotyping by image cytometry: Model studies using latex microbeads labeled with seven streptavidin-bound fluorochromes. Cytometry 24(3), 214-225 (1996).