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CFSE
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
CFSE图片
CAS NO:150347-59-4
包装与价格:
包装价格(元)
5 mg电议
10 mg电议
25 mg电议
50 mg电议
100 mg电议
200 mg电议
500 mg电议
1 mL*10 mM(in DMSO)电议

产品名称
5(6)-CFDA N-succinmidyl ester
5(6)-Carboxyfluorescein diacetate succinimidyl ester
CFDA-SE
Carboxyfluorescein succinimidyl ester
5(6)-羧基二乙酸荧光素琥珀酰亚胺酯
产品介绍
CFSE 是细胞内荧光染料,能够追踪细胞增殖。共价结合的 CFSE 在子细胞之间平均分配,可用于区分连续的细胞分裂。

产品描述

Carboxyfluorescein succinimidyl ester (CFSE) is a fluorescent cell staining dye. CFSE is cell permeable and covalently couples, via its succinimidyl group, to intracellular molecules, notably, to intracellular lysine residues and other amine sources.

体外活性

The binding of CFDA-SE fluorescent dye to cells is stable. It is not toxic and does not modify the viability of labeled cells. CFDA-SE fluorescent dye is a suitable fluorochrome for cell labeling. CFDA-SE persists in cells 24 h after labeling, allowing the long-term detection of labeled cells[1].

体内活性

In vivo labeling of cells with CFSE is feasible with virtually no toxic effect on the labeled or adjacent cells. It has advantages in relatively long-term migration studies as demonstrated by the recovery of T-cells in the peripheral lymphoid organs[2].

细胞实验

The 5 mM CFDA-SE stock in DMSO is diluted to different concentrations(2 μM, 3 μM, 4 μM, 5 μM, 10 μM and 20 μM) in PBS with a total volume of 1 ml. After each cell line is harvested and washed three times with PBS, 1×109 cells are added to equal volume of CFDA-SE with different concentrations and incubated at 37°C for 5, 6, 7, 8, 10 and 15 min with agitation. The labeling reaction is stopped for 1 min by adding an equal volume of heat inactivated fetal bovine serum. The CFDA-SE labeled cells are washed twice with PBS and recounted, and the cell concentration is adjusted to 6×104cells/ml in IMDM containing 10% FCS.(Only for Reference)

Cas No.

150347-59-4

分子式

C58H38N2O22

分子量

1114.934

别名

5(6)-CFDA N-succinmidyl ester;5(6)-Carboxyfluorescein diacetate succinimidyl ester;CFDA-SE;Carboxyfluorescein succinimidyl ester;5(6)-羧基二乙酸荧光素琥珀酰亚胺酯

储存和溶解度

Ethanol:<1 mgml
DMSO:93 mg/mL (166.8 mM)
H2O:<1 mgml
Powder: -20°C for 3 years
In solvent: -80°C for 2 years