Columbianadin 是来自当归的根的主要生物活性成分之一，是一种天然香豆素，具有抗炎和抗癌作用。

Columbianadin, one of the main bioactive constituents of the roots of Angelica pubescens Maxim. f. biserrata Shan et Yuan, has been found to possess obvious pharmacological effects in previous studies.

Cells are treated with various concentrations of Dihydrotanshinone I (3.13-20 μM) for 48 h. For the activity assay, Ac-DEVD-AMC (1 μg/μL), Ac-IETD-AMC (1 μg/μL) or Ac-LEDH-AMC (1 μg/μL) and cell lysate are added into Protease Assay Buffer in 96-well plate. Reaction mixtures with lysis buffer are used as negative controls. Cells treated with DMSO (0.1%) are treated as vehicle control. The reaction mixtures are incubated for 1 h at 37°C. The AMC liberated from the substrates is measured using spectrofluorometer of Victor 2 plate reader with an excitation wavelength of 380 nm and an emission wavelength of 430 nm.

5058-13-9

C19H20O5

328.364

Zosimin;2-Butenoic acid;二氢欧山芹醇当归酸酯

Chloroform:Soluble
DMSO:59 mg/mL(179.7 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years