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Vinorelbine
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Vinorelbine图片
CAS NO:71486-22-1
包装与价格:
包装价格(元)
20 mg电议
1 mL*10 mM(in DMSO)电议

产品名称
KW-2307 base
长春瑞宾
产品介绍
Vinorelbine是一种半合成的长春花生物碱。它与微管蛋白结合并阻止有丝分裂纺锤体的形成,导致中期肿瘤细胞生长停滞。

产品描述

Vinorelbine is a semisynthetic vinca alkaloid. Vinorelbine binds to tubulin and prevents the formation of the mitotic spindle, resulting in the arrest of tumor cell growth in metaphase.

体外活性

Vinorelbine (0.5-5 nM) inhibits cell proliferation by 50% (IC50) at concentrations of 1.25 nM. At concentration of 8 nM vinorelbine, no cells are in anaphase[1]. Vinorelbine time-dependently induces the p53 and p21WAFI/CIP1 expression in androgen-dependent (AD) and- independent (AI) prostate cancer cell lines. Vinorelbine stimulates reporter genes in a concentration-dependent manner[2].

体内活性

After vinorelbine treatment, the first neutropenicepisode occurred after the first (4 dogs), second (1), or sixth(1) vinorelbine treatment in the dogs[3]. Vinorelbine is tolerated at a weekly interval in tumor-bearing cats, with an MTD of 11.5 mg/m2[4].

激酶实验

Reverse transcriptase assays: Reverse transcriptase assays are carried out in a reaction mixture (50 μL) containing 50 mM Tris-HCl (pH 7.5), 5 mM DTT, 100 mM potassium chloride, 0.01% Triton X-100 or NP40, 10 μg/ml (dT)15.(A)n as template primer and [3H] deoxythymidine triphospahate. The reaction mixture is incubated for 1 hr at 37 ℃ and stopped by the addition of 50 μg of yeast tRNA and 2 mL of 10% solution of trichloroacetic acid containing 1 mM sodium pyrophosphate. The samples are filtered on filters (0.45 μm), washed first with 5% TCA solution for 5 times and then with 2 ml of 70% ethanol. The filters are dried, scintillation fluid is added and the radioactivity counted in a counter.

Cas No.

71486-22-1

分子式

C45H54N4O8

分子量

778.947

别名

KW-2307 base;长春瑞宾

储存和溶解度

DMSO:100 mg/mL (128.38 mM)
H2O:10 mg/mL (12.84 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years