ROSamultin 是从Potentilla anserina L分离的 19 α-羟基型三萜。它抑制 HIV-1 蛋白酶,具有抗氧化、抗炎和镇痛作用。
产品描述
Rosamultin has antioxidant, antiinflammatory/antinociceptive properties,and has anti-human immunodeficiency virus (HIV) activity.
体外活性
Rosamultin isolated from the root of R. rugosa inhibited HIV-1 protease by 53% at a concentration of 100 microM.
激酶实验
A substrate, His-Lys-Ala-ArgVal-Leu-(pNO2-Phe)-Glu-Ala-Nle-Ser-NH2, Inc. For the HIV1 protease assay, the stock solution of the prepared enzyme was diluted. The extracts or compounds(Rosamultin) tested were dissolved in dimethyl sulfoxide (10% in the reaction mixture). A reaction mixture (5 uL) composed of 1 L of 50 uM sodium acetate (pH 5.0), 1 uL of a substrate solution, 1uL of the plant extract or compound solution, and 2 uL of HIV1 protease solution, was stirred, centrifuged, and then incubated at 37℃ for 1 hour in a microtube. A control reaction was performed under the same conditions without plant extract or compounds. The reaction was stopped by heating at 90 ℃ for 1 minute. Thirty-five microliters of autoclaved water was added into the reaction mixture, and an aliquot of 5 uL was analyzed by high-performance liquid chromatography (HPLC).
Cas No.
88515-58-6
分子式
C36H58O10
分子量
650.85
别名
罗莎白素;野蔷薇苷
储存和溶解度
DMSO:10 mM
Powder: -20°C for 3 years
In solvent: -80°C for 2 years