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FICZ
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
FICZ图片
CAS NO:172922-91-7
包装与价格:
包装价格(元)
1 mg电议
5 mg电议
10 mg电议
25 mg电议
50 mg电议
1 mL*10 mM(in DMSO)电议

产品名称
6-Formylindolo[3,2-b]carbazole
产品介绍
FICZ是芳烃受体 (AhR) 的有效芳基激动剂,Kd值为70 pM。

产品描述

FICZ is a potent aryl agonist for the hydrocarbon receptor (AhR, Kd: 70 pM).

体外活性

FICZ (0.01 nM-1 μM) alone or in combination with 50 nM MNF induces sustained CYP1A1 activity and leads to oxidative stress and activation of apoptosis via a mitochondrial-dependent pathway. In HepG2 cells, FICZ stimulates cell growth at low concentrations but inhibits cell growth at high concentrations [1]. FICZ (10-30 μM) significantly decreases CEH viability with an estimated LC50 (95% confidence intervals) of 14 μM. FICZ shows concentration-dependent effects on EROD activity in CEH cultures, with the mean EC50 values at 3, 8, and 24 h of 0.016 nM, 0.80 nM, and 11 nM, respectively [2]. CYP1 inhibition in the presence of FICZ results in enhanced AHR activation, suggesting that FICZ accumulates in the cell when its metabolism is blocked. CYP1 enzymes play a role in regulating the biological effects of FICZ [3]. FICZ treatment increases transcript expression of CYP1A1 in a dose-dependent manner in both the parental iPSC line and the CYP1A1 targeted clone [4].

细胞实验

The cell viability of CEH treated with FICZ or TCDD is studied with the untreated cells (used as a live cell control) and sodium hypochlorite (5%)-treated cells (used as a dead cell control). This assay is based upon the bioluminescent measurement of adenosine triphosphate (ATP) that is present in all metabolically active cells. Luciferase is utilized in this method to catalyze the formation of light from ATP and luciferin. CEH is lysed 24 h after dosing and the luminescence emitted from the ATP-dependent oxidation of luciferin is measured with a LuminoSkan Ascent luminometer [2].

Cas No.

172922-91-7

分子式

C19H12N2O

分子量

284.31

别名

6-Formylindolo[3,2-b]carbazole

储存和溶解度

DMSO:10 mg/mL (35.17 mM),Need ultrasonic and warming
Powder: -20°C for 3 years
In solvent: -80°C for 2 years