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Z-DEVD-FMK
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Z-DEVD-FMK图片
CAS NO:210344-95-9
包装与价格:
包装价格(元)
1 mg电议
5 mg电议
10 mg电议
1 mL*10 mM(in DMSO)电议

产品名称
Caspase-3 Inhibitor
产品介绍
Z-DEVD-FMK 是一种选择性、不可逆的 Caspase-3 抑制剂,IC50为 18 μM。 它也抑制 Caspase-6、Caspase-7、Caspase-8 和 Caspase-10。

产品描述

Z-DEVD-FMK is a selective, irreversible Caspase-3 inhibitor, and also exhibits effective inhibition activity on caspase-6, caspase-7, caspase-8, and caspase-10.

体内活性

Z-DEVD-FMK, before and after injury, markedly reduces post-traumatic apoptosis, and significantly improved neurological recovery. [2]

激酶实验

Caspase activity assay : Caspase-3 and caspase-9 activities are measured using fluorescent-based substrate. After treatment, the cells are resuspended in lysis buffer (50 mM Tris HCl, 1 mM EDTA, and 10 mM EGTA) containing 10 mM digitonin for 20 min at 37°C. Supernatants are treated with either of the fluorogenic substrates Ac-DEVD-AFC for caspase-3 or Ac-LEHD-AFC for caspase-9 for 1 h at 37°C and fluorescence is measured at excitation at 400 nm and emission at 505 nm using a Gemini XS fluorescence plate reade

细胞实验

N27 cells are incubated with 100 μM 6-OHDA for 24 h or 300 μM MPP+ for 36 h in the presence or absence of 50 μM Z-DEVD-FMK and cell death is determined by MTT (3-(4,5-dimethylthiazol-3-yl)-2,5-diphenyl tetrazolium bromide) assay, which is widely used to assess cell viability. After treatment, the cells are incubated in serum-free medium containing 0.25 mg/ml MTT for 3 h at 37°C. Formation of formazan from tetrazolium is measured at 570 nm with a reference wavelength at 630 nm using a SpectraMax microplate reader.(Only for Reference)

Cas No.

210344-95-9

分子式

C30H41FN4O12

分子量

668.672

别名

Caspase-3 Inhibitor

储存和溶解度

DMSO:93 mg/mL (139.1 mM)
H2O:<1 mgml
Ethanol:<1 mgml
Powder: -20°C for 3 years
In solvent: -80°C for 2 years