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Ac-DEVD-AMC
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Ac-DEVD-AMC图片
CAS NO:169332-61-0
包装与价格:
包装价格(元)
1 mg电议
5 mg电议
10 mg电议
25 mg电议
50 mg电议
100 mg电议
200 mg电议
500 mg电议
1 mL*10 mM(in DMSO)电议

产品名称
AC-ASP-MET-GLN-ASP-7-AMINO-4-METHYLCOUMARIN
AC-ASP-MET-GLN-ASP-AMC
产品介绍
Ac-DEVD-AMC 是一种Caspase-3底物。

产品描述

Ac-DEVD-AMC is the substrate of Caspase-3 .

体外活性

The AMC moiety is highest at 2 h with an area?of 5.6±1.8, the AMC peak area for Ac-DEVD-AMC incubated without a lung specimen (substrate alone) for 6 h is relatively small [1]. Ac-DEVD-AMC can be used to monitor intracellular caspase-3 activity[2].

细胞实验

Lung fragments (about 20 mg each) are collected from rats and mice. The samples are incubated at 37°C in 50 mL KH buffer or MEM (continuously gass with 95% O2: 5% CO2) for up to 6 h. At specific time points, samples are incubated in oxygenated KH buffer or MEM with 32 μM zVAD-fmk or 15 μL DMSO for 20 min (f/v=1.0 mL). Ac-DEVD-AMC (37 μM) is then added and the incubation continues for an additional 20 min. At the end of incubation, the tissue is disrupted by vigorous homogenization for 2 min, sonication for 3 min, and 10 passages through a 27-G needle. This disruption procedure quenches the Ac-DEVD-AMC cleavage reaction due to dilution. The supernatants are collected by centrifugation (~6,300g for 90 min) through a microcentrifuge filter, separated on HPLC, and analyzed for the fluorogenic AMC moiety [2].

Cas No.

169332-61-0

分子式

C30H37N5O13

分子量

675.64

别名

AC-ASP-MET-GLN-ASP-7-AMINO-4-METHYLCOUMARIN;AC-ASP-MET-GLN-ASP-AMC

储存和溶解度

DMSO:≤10mg/ml(14.80 mM)
H2O:< 0.1 mg/mL (insoluble)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years