PTP1B-IN-2 是一种蛋白酪氨酸磷酸酶抑制剂,IC50=50 nM。
产品描述
PTP1B-IN-2 is an effective protein tyrosine phosphatase 1B (PTP1B) inhibitor(IC50=50 nM).
体外活性
PTP1B-IN-2 extends deep into the active site pocket, forming several hydrogen bonds and hydrophobic interactions with key residues of the catalytic site. PTP1B-IN-2 greatly enhances insulin-mediated IRβ phosphorylation at concentrations of 15 μM and 30 μM.
激酶实验
The PTP1B enzymatic assay, the total volume of 100 μL per well contains15 nM recombinant PTP1B protein, 2 mM p-nitrophenylphosphonic acid (pNPP), 1 mM dithiothreitol and 1 mM EDTA (pH 6.5). After 30 min incubation at 37℃, end the reaction by addition of 2.5 M NaOH. The hydrolysis product, pNP, is detected at the absorbance at 405 nm.
Cas No.
1919853-46-5
分子式
C34H36N2O9S2
分子量
680.79
储存和溶解度
DMSO:100 mg/mL
Powder: -20°C for 3 years
In solvent: -80°C for 2 years