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N6-Methyladenosine
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
N6-Methyladenosine图片
CAS NO:1867-73-8
包装与价格:
包装价格(元)
50 mg电议
100 mg电议
200 mg电议
500 mg电议

产品名称
N-Methyladenosine
6-Methyladenosine
N6-甲基腺苷
m6A
NSC-29409
产品介绍
N6-Methyladenosine 是甲基化的腺嘌呤残基,苷是转移核糖核酸降解的内源性尿核苷产物。它是存在于所有高等真核生物中最普遍的的信使 RNA 内部修饰,可修饰病毒 RNA,有抗病毒活性。

产品描述

N6-Methyladenosine is a methylated adenine residue. N6-Methyladenosine is an endogenous urinary nucleoside product of the degradation of transfer ribonucleic acid (tRNA); urinary nucleosides are biological markers for patients with colorectal cancer.

体外活性

N6-Methyladenosine is an abundant modification in mRNA and is found within some viruses, and most eukaryotes including mammals, insects, plants and yeast. It is also found in tRNA, rRNA, and small nuclear RNA(snRNA) as well as several long non-coding RNA, such as Xist. [1] [2] [3] N6-Methyladenosine is an endogenous urinary nucleoside product of the degradation of transfer ribonucleic acid (tRNA). [4] N6-Methyladenosine is a widespread RNA modification in many tissues with high levels in the brain. N6-Methyladenosine is enriched near stop codons and within 3'UTRs in both mouse and human mRNAs. [5] The recent discovery that FTO, an obesity risk gene, encodes an m6A demethylase implicates m6A as an important regulator of physiological processes. [6]

体内活性

LD50: Mice >1 g/kg (i.g.). [7]

激酶实验

NOD1 Dose Response assay: Day 1 Procedure 1) Harvest HEK-293-T NFKB-Luc at 100% confluency at 100% confluency. 2) Add NOD assay media with Multidrop. 3) Spin down plates at 1000 rpm for 1 min in centrifuge. 4) Serial compound dilutions. 5) Add gamma-tri-DAP to cell suspension at 0.75 ug/mL. 6) Seed 13000 cells/well in 4 uL/well to full plate HEK-293-T NFKB-Luc to TC-treated plate. 7) Spin down plates 500 RPM for 5 min on centrifuge. 8) Lid Plates. Sandwich 4 plates between 2 lidded 384 plates filled with Water. 9) Wrap plates securely in single layer of Plastic Wrap. 10) Incubate overnight (14 hours) in 37 ℃ and 5% CO2 incubator. Day 2 Procedure 1) Add 3 ul/well of SteadyGlo solution with Multidrop. 2) Shake plates on a plate shaker for 20 min. 3) Spin plates 1000 RPM for 1 min using centrifuge. 4) Read luminescence.IC50 values are calculated using GraphPad Prism 5.0.The average Z' for the screen is 0.6, the signal to background is 11.1, signal to noise is 78.6 and signal to window is 6.0.

Cas No.

1867-73-8

分子式

C11H15N5O4

分子量

281.272

别名

N-Methyladenosine;6-Methyladenosine;N6-甲基腺苷;m6A;NSC-29409

储存和溶解度

H2O:14 mg/mL (49.77 mM)
Ethanol:2 mg/mL (7.11 mM)
DMSO:56 mg/mL (199.09 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years