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Nucleozin
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Nucleozin图片
CAS NO:341001-38-5
包装与价格:
包装价格(元)
2 mg电议
5 mg电议
10 mg电议
25 mg电议
50 mg电议
100 mg电议
1 mL*10 mM(in DMSO)电议

产品名称
[4-(2-氯-4-硝基苯基)-1-哌嗪基](5-甲基-3-苯基-4-异噁唑基)-甲酮
产品介绍
Nucleozin 靶向流感 A 核蛋白,这是一种病毒复制所必需的多功能 RNA 结合蛋白。

产品描述

Nucleozin targets influenza A nucleoprotein (NP), a multifunctional, RNA-binding protein necessary for virus replication.

体外活性

Nucleozin blocked the cytoplasmic trafficking of ribonucleoproteins (RNPs) that had undergone nuclear export, promoting the formation of large perinuclear aggregates of RNPs along with cellular Rab11. This effect led to the production of much reduced amounts of often markedly smaller virus particles. The primary target of nucleozin is the viral RNP. IAV replication can be effectively inhibited by blocking cytoplasmic trafficking of the viral genome[1].

体内活性

Nucleozin, that triggers the aggregation of NP and inhibits its nuclear accumulation. Nucleozin impeded influenza A virus replication in vitro with a nanomolar median effective concentration (EC(50)) and protected mice challenged with lethal doses of avian influenza A H5N1. Viral NP is a valid target for the development of small-molecule therapies[2].

细胞实验

For live imaging, cells were grown in chambered glass bottom dishes and maintained at 37°C in L-15 CO2-independent medium (Gibco) during analysis. Cells were transfected with GFP-NP and infected with PR8 12 h later. For nucleozin treatment, images were acquired for 5 min and then nucleozin was added to a final concentration of 2 μM before imaging for around 20 to 30 min . Images were acquired at 0.25 or 0.71 frame/s and processed with ImageJ[1].

Cas No.

341001-38-5

分子式

C21H19ClN4O4

分子量

426.86

别名

[4-(2-氯-4-硝基苯基)-1-哌嗪基](5-甲基-3-苯基-4-异噁唑基)-甲酮

储存和溶解度

DMSO:28.66 mg/mL (67.14 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years