Nelfinavir Mesylate是一种口服活性的HIV-1蛋白酶抑制剂，用于抑制 HIV感染，是一种广谱抗癌剂。

Nelfinavir Mesylate is an effective HIV protease inhibitor (Ki: 2 nM).

GTPγ[35S] binding assay: The cells are homogenized and centrifuged at 26900 × g for 30 min at 4°C. Membranes are re-suspended in 20 mM HEPES (pH 7.4), 100 mM NaCl, 10 mM MgCl2, 1 mM EDTA and 0.1% fat-free BSA at 2–3 mg protein/mL. GTPγ[35S] binding assay is performed with the membranes (75 mg protein /mL in 50 mM HEPES, 100 mM NaCl, 10 mM MgCl2, 20 μg/mL saponin and 0.1% fat-free BSA (pH 7.4), 5 mg/mL with wheat-germ agglutinin-coated scintillation proximity assay-bead, and 10 μM GDP for 10–15 min. The GTPγ[35S]-binding reaction is started by the addition of 200 pM GTPγ[35S]. After 120 min at room temperature, the plates are centrifuged for 10 min at 300 × g and counted.

159989-65-8

C33H49N3O7S2

663.89

AG1343;AG 1343 Mesylate;Nelfin;甲磺酸奈非那韦;Viracept

H2O:<1 mgml
DMSO:93 mg/mL (140.1 mM)
Ethanol:93 mg/mL (140.1 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years