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Nitroxoline
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Nitroxoline图片
CAS NO:4008-48-4
包装与价格:
包装价格(元)
500 mg电议
1 g电议
1 mL*10 mM(in DMSO)电议

产品名称
硝羟喹啉
8-Hydroxy-5-nitroquinoline
5-Nitro-8-hydroxyquinoline
5-nitroquinolin-8-ol
5-Nitro-8-quinolinol
产品介绍
Nitroxoline是一种抗生素,防治生物膜感染。它通过从生物膜基质螯合Fe2+和Zn2+离子而发挥作用。

产品描述

Nitroxoline is a urinary antibacterial agent active against susceptible gram-positive and gram-negative organisms commonly found in urinary tract infections.

体外活性

Nitroxoline significantly reduces extracellular DQ-collagen IV degradation by all evaluated cancer cell lines using spectrofluorimetry. Nitroxoline also markedly decreases tumor cell invasion monitored in real time and reduces the invasive growth of multicellular tumor spheroids, used as a 3D in vitromodel of tumor invasion. Endothelial tube formation is significantly reduced by nitroxoline in an in vitro angiogenesis assay[1].

体内活性

Nitroxoline significantly abrogates tumor growth, angiogenesis and metastasis in vivo in LPB fibrosarcoma and MMTV-PyMT breast cancer mouse models[1].

激酶实验

In Vitro Kinase Assays : The potency of SP600125 towards kinases, including MPS1, JNK, and Aurora kinase A, is determined based on the specific measurement of radioactive phosphotransfer to the substrate. For each enzyme, the absolute Km values for ATP and the specific substrate are initially determined and each assay is then run at optimized [ATP] (2·αKm) and [substrate] (5·Km) concentrations. MPS1 activity is measured using 5 nM of MPS1 recombinant protein in 50 mM HEPES pH 7.5, 2.5 mM MgCl2, 1 mM MnCl2, 1 mM DTT, 3 μM NaVO3, 2 mM β-glycerophosphate, 0.2 mg/mL BSA, 200 μM P38-βtide substrate-peptide (KRQADEEMTGYVATRWYRAE), and 8 μM ATP with 1.5 nM 33P-γ-ATP. Ten serial 1:3 dilutions (from 30 μM to 1.5 nM) of SP600125 are tested and IC50 determined.

细胞实验

cell suspension are seeded in the wells of an E-plate 16 according to the manufacturer's instructions. After seeding, the CI is monitored every 15 min. After ∼10 h (MCF-10A neoT and MMTV-PyMT), 14 h (U-87 MG) or 24 h (LPB), when the cells are in their log phase of growth, 50 μl of the compound or 0.1% DMSO is added, and the experiment allowed to run for 72 h. Once every 24 h the medium is replaced with fresh medium containing the inhibitor or suitable control to prevent cell death due to medium depletion. Compounds and their concentrations are: nitroxoline (5 μM) and CA-074 (5 μM) for all cell lines other than MCF-10A neoT cell line, where nitroxoline was used at 2.5 μM. All measurements were performed in quadruplicate. (Only for Reference)

Cas No.

4008-48-4

分子式

C9H6N2O3

分子量

190.158

别名

硝羟喹啉;8-Hydroxy-5-nitroquinoline;5-Nitro-8-hydroxyquinoline;5-nitroquinolin-8-ol;5-Nitro-8-quinolinol

储存和溶解度

Ethanol:<1 mgml
H2O:<1 mgml
DMSO:36 mg/mL (189.3 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years