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N1-Methylpseudouridine
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
N1-Methylpseudouridine图片
CAS NO:13860-38-3
包装与价格:
包装价格(元)
10 mg电议
25 mg电议
50 mg电议
1 mL*10 mM(in DMSO)电议

产品名称
1-Methylpseudouridine
N1-甲基-假尿苷
产品介绍
N1-Methylpseudouridine 是一种甲基假尿苷,翻译性能比 5 mC 和 5 mC/N1-Methylpseudouridine高。通过提高核糖体密度,mRNA 中的 N1-Methylpseudouridine 以 eIF2α 依赖性和独立机制增强翻译。

产品描述

N1-Methylpseudouridine is a methylpseudouridine and enhances translation through eIF2α-dependent and independent mechanisms by increasing ribosome density in mRNA.

体外活性

In HEK293T cells, Incorporation of N1-Methylpseudouridine into mRNA modifies mRNAs produced higher amounts of luc than the standard Luc mRNA .?Incorporation of N1-Methylpseudouridine nucleoside modification in both Luc and GFP mRNA enhances the initiation step of translation, in part by suppressing eIF2α phosphorylation.?In addition, polysome formation and growth on the N1-Methylpseudouridine-containing Luc mRNA is enhanced due to the reduction of elongation rate[1].

体内活性

mRNAs containing the N1-Methylpseudouridine modification alone and/or in combination with 5-methylcytidine (m5C) outperformed the current state-of-the-art pseudouridine (Ψ) and/or m5C/Ψ-modified mRNA platform by providing up to ~44-fold (when comparing double modified mRNAs) or ~13-fold (when comparing single modified mRNAs) higher reporter gene expression upon transfection into cell lines or mice, respectively. We show that (m5C/)N1-Methylpseudouridine-modified mRNA resulted in reduced intracellular innate immunogenicity and improved cellular viability compared to (m5C/)Ψ-modified mRNA upon in vitro transfection[2].

Cas No.

13860-38-3

分子式

C10H14N2O6

分子量

258.23

别名

1-Methylpseudouridine;N1-甲基-假尿苷

储存和溶解度

H2O:40 mg/mL (154.90 mM)
DMSO:100 mg/mL (387.25 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years