Methoxychlor 是一种有机氯农药，被认为是一种内分泌干扰物，会影响不同细胞模型中的 Ca2? 稳态和细胞活力。

Methoxychlor, an organochlorine pesticide, is thought to be an endocrine disrupter that affects Ca2 homeostasis and cell viability in different cell models.

In HA59T cells, methoxychlor induced a [Ca2?]i rise by inducing Ca2? entry via protein kinase C-sensitive Ca2?-permeable channels, without causing Ca2? release from stores. Methoxychlor also induced apoptosis that was independent of [Ca2?]i rises.

Fura-2, a Ca2?-sensitive fluorescent dye, was applied to measure [Ca2?]i. Methoxychlor at concentrations of 0.1-1 μM caused a [Ca2?]i rise in a concentration-dependent manner. Removal of external Ca2? abolished methoxychlor's effect. Methoxychlor-induced Ca2? influx was confirmed by Mn2?-induced quench of fura-2 fluorescence. Methoxychlor-induced Ca2? entry was inhibited by nifedipine, econazole, SK&F96365, and protein kinase C modulators. Methoxychlor killed cells at concentrations of 10-130 μM in a concentration-dependent fashion. Chelation of cytosolic Ca2? with 1,2-bis(2-aminophenoxy) ethane-N,N,N',N'-tetraacetic acid/AM (BAPTA/AM) did not prevent methoxychlor's cytotoxicity. Methoxychlor (10 and 50 μM) induced apoptosis concentration-dependently as determined by using Annexin V/propidium iodide staining

72-43-5

C16H15Cl3O2

345.64

Methoxychlor

DMSO:10 mM
Powder: -20°C for 3 years
In solvent: -80°C for 2 years