FH1 可提高肝细胞功能，促进诱导多能干 (iPS) 衍生的肝细胞向更成熟的表型分化，并促使分化良好的肝细胞样细胞 (iHeps) 培养物的成熟。

FH1 (BRD-K4477), a small molecule, enhances the functions of hepatocytes and promotes differentiation hepatocytes derived by iPS.

在肝细胞中,FH1(BRD-K4477)促进分化完整的iHeps成熟,增强细胞功能。

Affinity determination: Binding affinities (Ki or IC50) of ABT-263 against different isoforms of Bcl-2 family are determined with competitive fluorescence polarization assays. The following peptide probe/protein pairs are used: f-bad (1 nM) and Bcl-xL (6 nM), f-Bax (1 nM) and Bcl-2 (10 nM), f-Bax (1 nM) and Bcl-w (40 nM), f-Noxa (2 nM) and Mcl-1 (40 nM), and f-Bax (1 nM) and Bcl-2-A1 (15 nM). Binding affinities for Bcl-xL are also determined using a time-resolved fluorescence resonance energy transfer assay. Bcl-xL (1 nM, His tagged) is mixed with 200 nM f-Bak, 1 nM Tb-labeled anti-His antibody, and ABT-263 at room temperature for 30 min. Fluorescence is measured on an Envision plate reader using a 340/35 nm excitation filter and 520/525 (f-Bak) and 495/510 nm (Tb-labeled anti-His antibody) emission filters.

2719-05-3

C17H18N2O2

282.343

BRD-K4477;NSC 12407;FH1

Ethanol:<1 mgml
DMSO:52 mg/mL (184.2 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years