ITX3 is a specific and nontoxic inhibitor of the TrioN (N-terminal GEF domain of the multidomain Trio protein) (IC50: 76 uM). ITX3 inhibits TrioN-stimulated RhoG exchange in vitro.

TrioN inhibitor In transfected mammalian cells, ITX3 blocked TrioN-mediated dorsal membrane ruffling and Rac1 activation while having no effect on GEF337-, Tiam1-, or Vav2-mediated RhoA or Rac1 activation. ITX3 specifically inhibited endogenous TrioN activity, as evidenced by its ability to inhibit neurite outgrowth in nerve growth factor (NGF)–stimulated PC12 cells or C2C12 differentiation into myotubes [1]. ITX3 repressed the Rac1 activity and dose-dependently up-regulated the E-cadherin protein level in the Tara-KD cells [2].

347323-96-0

C22H17N3OS

371.46

ITX3

DMSO:2 mg/mL (5.38 mM),ultrasonic and warming and heat to 80°C
Powder: -20°C for 3 years
In solvent: -80°C for 2 years