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IC-87114
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
IC-87114图片
CAS NO:371242-69-2
包装与价格:
包装价格(元)
2 mg电议
5 mg电议
10 mg电议
50 mg电议
100 mg电议
200 mg电议

产品名称
IC 87114
产品介绍
IC87114 是一种有效的、选择性的PI3Kδ抑制剂,其 IC50=0.5 μM。

产品描述

IC-87114 is a specific PI3Kδ inhibitor(IC50=0.5 μM).

体外活性

在有炎症反应的鼠模型中,IC87114阻断TNF1α刺激产生蛋白酶的胞外分泌.

体内活性

在人类中性粒细胞中,IC87114(5 μM)能够地抑制N-甲酰 - 甲硫氨酰 - 亮氨酰 - 苯丙氨酸(fMLP) - 刺激的磷脂酰肌醇三磷酸(PIP3)的生物合成和趋化性。在人类急性骨髓性白血病(AML)原始细胞如骨髓单个核细胞(BMMCs)中,IC87114(10 μM)抑制组成型和Flt-3刺激的Akt磷酸化和细胞增殖。在抗CD3刺激的小鼠CD62L +(幼稚)和CD62L-(效应/记忆)CD4 + T细胞中,IC87114抑制增殖和干扰素-γ(IFN-γ)产生。

激酶实验

PI3K kinase assay: Phosphatidylinositol-(4,5)-bisphosphate (PIP2) containing phospholipid liposomes are prepared. Briefly, bovine PIP2 and phosphatidylserine (1:2 molar ratio) are vacuum-dried and resuspended at 1 mM PIP2 in 20 mM HEPES-KOH, pH 7.4, 50 mM NaCl, and 5 mM EDTA. The lipid suspension is subjected to a brief sonication, followed by 5 freeze-thaw cycles and then 20 extrusion cycles to produce the liposomes. The assay is conducted in 60 μL reaction volumes in 20 mM HEPES, pH 7.4, buffer containing 1 nM PI3K, 1 μM PIP2, 200 μM ATP, 1 μCi [γ-32P]ATP, 5 mM MgCl2, plus 50 μg/mL horse IgG as carrier protein. The reaction is incubated for 10 min at room temperature, quenched in 140 of 1 M K2PO4, 30 mM EDTA, pH 8.0, captured onto a 96-well polyvinylidene difluoride filter plate, and washed five times with 1 M K2PO4. The filter is allowed to dry completely, and the bound radioactivity is quantitated. IC87114 dilutions are assayed in a final concentration of 1% (w/w) DMSO.

细胞实验

For AML cell proliferation assay, BMMCs are isolated and cultured in α-medium with 5% fetal calf serum (FCS) with or without FLT-3 ligand (10 ng/mL) for 48 hours and with or without IC87114. [3H]-thymidine (1 μCi [37 kBq]) is added for a final 6 hours and the amount of radioactivity incorporated is determined by trichloracetic acid precipitation. CD34+ cells from cord blood are cultured in stem cell factor (SCF; 20 ng/mL), FLT-3 ligand (10 ng/mL), and Tpo (20 nM) for 48 hours with or without 10 μM IC87114 and pulsed for 12 hours with [3H]-thymidine. (Only for Reference)

Cas No.

371242-69-2

分子式

C22H19N7O

分子量

397.442

别名

IC 87114;IC-87114

储存和溶解度

DMSO:10 mg/mL (25.16 mM),Need ultrasonic
Powder: -20°C for 3 years
In solvent: -80°C for 2 years