Reactive oxygen species (ROS) play important roles in the initiation and progression of many disease processes. Most fluorescent probes for ROS detection, such as 2,7-dichlorofluorescein (DCFH), function by an oxidative mechanism and are useful for total oxidant detection but are not selective for hydrogen peroxide specifically. Pentafluorobenzenesulfonyl fluorescein is a fluorescent probe that functions by a nonoxidative mechanism. It fluoresces upon perhydrolysis of the sulfonyl linkage, and is selective for hydrogen peroxide over hydroxyl radical, tert-butyl hydroperoxide, superoxide anion, singlet oxygen, and nitrates. Pentafluorobenzenesulfonyl fluorescein can be used as a probe for hydrogen peroxide in whole cell systems.

728912-45-6

C26H11F5O7S

562.42

Pentafluorobenzenesulfonyl fluorescein

Ethanol:20 mg/mL
DMF:30 mg/mL
DMSO:30 mg/mL
DMSO:PBS (pH 7.2)(1:1):0.25 mg/mL
Powder: -20°C for 3 years
In solvent: -80°C for 2 years