7β,27-dihydroxy Cholesterol is an oxysterol and agonist of retinoic acid receptor-related orphan receptor γ (RORγ) and RORγt. [1] It activates RORγ- or RORγt-dependent signaling with EC50 values of 691 and 1,045 nM, respectively, in reporter assays using HEK293T cells expressing the recombinant human receptors. 7β,27-dihydroxy Cholesterol is selective for RORγ and RORγt over a panel of eight additional nuclear receptors at 30 µM. It increases IL-17A production in Th17-polarized isolated human na ve CD4+ T cells when used at a concentration of 300 nM. 7β,27-dihydroxy Cholesterol (60 mg/kg) increases IL-17A production in isolated mouse γδ T cells stimulated with 12-myristate 13-acetate and ionomycin .

240129-43-5

C27H46O3

418.65

7β,27-dihydroxy Cholesterol;7β,27-DHC;7β,27-dihydroxy Cholesterol

Ethanol:PBS (pH 7.2) (1:2):0.30 mg/mL
DMF:2 mg/mL
Ethanol:20 mg/mL
Powder: -20°C for 3 years
In solvent: -80°C for 2 years