Phosphatase Inhibitor Cocktail I (100× ddH2O)
产品优势
- 组分清晰,浓度明确;
- 产品兼容性好,高效抑制多种磷酸酶,全面保护蛋白的磷酸化状态;
产品应用
在室温下解冻,开盖前请低速离心一下,以便将黏附于管壁的液体甩至管底。然后在实验前以1:100(v / v)稀释至溶液样品(如细胞裂解液或组织提取液),短暂涡旋震荡加速溶解。应用于:WB、IP、Co-IP、pull-down、IF、IHC、kinase assay等。
产品组成
Ingredient | CAS | MW | Concentration (100x) | Target | Type |
---|
Sodium Fluoride | 7681-49-4 | 41.99 | 100 mM | Acid Phosphatase | Reversible |
Sodium Orthovanadate | 13721-39-6 | 183.91 | 100 mM | Alkaline phosphatase, PTPs, ATPases | Reversible |
Sodium Molybdate | 7631-95-0 | 205.92 | 115 mM | Acid and phosphoprotein Phosphatase | Irreversible |
Sodium Tartrate | 868-18-8 | 194.05 | 400mM | Acid Phosphatase | Reversible |
Imidazole | 288-32-4 | 68.08 | 200 mM | Alkaline phosphatase | Reversible |