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Indirubin(NSC 105327 Couroupitine B)
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Indirubin(NSC 105327 Couroupitine B)图片
CAS NO:479-41-4
规格:≥98%
包装与价格:
包装价格(元)
5mg电议
25mg电议
50mg电议
100mg电议
250mg电议
500mg电议
1g电议

产品介绍
理化性质和储存条件

Molecular Weight (MW)

262.26

Formula

C16H10N2O2

CAS No.

479-41-4

Storage

-20℃ for 3 years in powder form

-80℃ for 2 years in solvent

Solubility (In vitro)

DMSO: 53 mg/mL (202.1 mM)

Water: <1 mg/mL

Ethanol: <1 mg/mL

Other info

Chemical Name: 3-(1,3-Dihydro-3-oxo-2H-indol-2-ylidene)-1,3-dihydro-2H-indol-2-one

InChi Key: CRDNMYFJWFXOCH-YPKPFQOOSA-N

InChi Code: InChI=1S/C16H10N2O2/c19-15-10-6-2-4-8-12(10)17-14(15)13-9-5-1-3-7-11(9)18-16(13)20/h1-8,17H,(H,18,20)/b14-13-

SMILES Code: O=C1NC2=C(C=CC=C2)/C1=C3NC4=C(C=CC=C4)C/3=O

Synonyms

NSC 105327, Couroupitine B; C.I. 73200, Indigo red, Indigopurpurin; NSC-105327, NSC 105327; NSC105327.

实验参考方法

In Vitro

In vitro activity: Indirubin is the active ingredient of Danggui Longhui Wan, a mixture of plants that is used in traditional Chinese medicine to treat chronic diseases. Indirubin inhibits CDKs activity with IC50 of 2.2 - 10 μM resulting in cell cycle arrest in the G2/M phase. Indirubin also inhibits GSK-3β with an IC50 of 0.6 μM, attenuating CDK5- and GSK-3β-mediated tau phosphorylation, a process over-active in Alzheimer disease states. It also suppresses tumor necrosis factor (TNF)-induced NF-κB activation in a dose- and time-dependent manner. Indirubin also suppresses the NF-κB activation induced by various inflammatory agents and carcinogens. Indirubin blocks the phosphorylation and degradation of IκBα through the inhibition of activation of IκBα kinase and phosphorylation and nuclear translocation of p65.

Cell Assay: Indirubin exerted its inhibitory effects not only on interferon-γ production by human myelomonocytic HBL-38 cells but also on interferon-γ and interleukin-σ production by murine splenocytes with no influence on the proliferation of either cells.

In Vivo

Because of Indirubin’s inhibitory activity on interferon-γ production, the authors further investigated the effects of indirubin on 2,4,6-trinitro-l-chlorobenzene-induced delayed-type hypersensitivity. When injected intraperitoneally, indirubin significantly inhibited the ear swelling of TNCB-elicited mice. Moreover,the amount of interferon-γ in the culture supernatants of elicited mouse lymphocytes was inhibited by indirubin treatment

Animal model

Mice

Formulation & Dosage

i.p.

References

J Biol Chem. 2006 Aug 18;281(33):23425-35; J Biol Chem. 2001 Jan 5;276(1):251-60.