您好,欢迎来到化工原料网! [登录] [免费注册]
化工原料网
位置:首页 > 产品库 > NCT-501
立即咨询
咨询类型:
     
*姓名:
*电话:
*单位:
Email:
*留言内容:
请详细说明您的需求。
*验证码:
 
NCT-501
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
NCT-501图片
CAS NO:1802088-50-1
规格:≥98%
包装与价格:
包装价格(元)
10mg电议
25mg电议
50mg电议
100mg电议
250mg电议
500mg电议

产品介绍
理化性质和储存条件
Molecular Weight (MW)416.52
FormulaC21H32N6O3
CAS No.1802088-50-1
Storage-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)DMSO: 32 mg/mL (76.8 mM)
Water: <1 mg/mL
Ethanol: 25 mg/mL (60.0 mM)
SMILES O=C(C(N(CCC(C)C)C(CN1CCN(C(C2CC2)=O)CC1)=N3)=C3N4C)N(C)C4=O
Synonyms NCT501; NCT 501; NCT-501; 8-((4-(cyclopropanecarbonyl)piperazin-1-yl)methyl)-7-isopentyl-1,3-dimethyl-3,7-dihydro-1H-purine-2,6-dione
实验参考方法
In Vitro

In vitro activity: In Caco-2 cell line, NCT-501 exhibits excellent permeability.


Kinase Assay: NCT-501 is a novel, highly potent and selective inhibitor of Aldehyde Dehydrogenase 1A1 (ALDH1A1) with IC50 of 40 NM. NCTAldehyde dehydrogenases (ALDHs) metabolize reactive aldehydes and possess important physiological and toxicological functions in areas such as CNS, metabolic disorders, and cancers. Increased ALDH (e.g., ALDH1A1) gene expression and catalytic activity are vital biomarkers in a number of malignancies and cancer stem cells, highlighting the need for the identification and development of small molecule ALDH inhibitors.


Cell Assay: For more than 450 human kinases, NCT-501 treatment at 10 μM inhibited ≥55% activity of these kinases. NCT-501 reversibly inhibited ALDH1A1.The plasma exposure for intraperitoneal administration (ip) at 30 mg/kg showed better than the oral administration (p.o) at 30 mg/kg. The values of AUC 24h were 5670 h·ng/mL and 484 h·ng/mL along with 100% and 29% bioavailability, respectively. The t1/2 was shorter than 1h. The clearance level was 98 mL/min/kg. These results together indicated that NCT-501 was well absorbed and distributed but rapidly metabolized or excreted. In vitro stability of NCT-501in CD1 mouse plasma was more than 60 min.

In VivoNCT-501 (100 μg/animal; i.t.; every alternate day for 20 days) shows a 78% inhibition in tumor growth in Cal-27 CisR derived xenografts.
Animal modelMale Hsd: Athymic Nude-Foxn1nu (immuno-deficient-mice bearing Cal-27 CisR cells)
Formulation & DosageIntra-tumorally (i.t); 100μg/animal
ReferencesJ Med Chem. 2015 Aug 13;58(15):5967-78.; Mol Carcinog. 2016 Jul 6.