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Indoximod(NLG 8189)
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Indoximod(NLG 8189)图片
CAS NO:110117-83-4
规格:≥98%
包装与价格:
包装价格(元)
250mg电议
500mg电议
1g电议
2g电议
5g电议

产品介绍
理化性质和储存条件
Molecular Weight (MW)218.25
FormulaC12H14N2O2
CAS No.110117-83-4
Storage-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)DMSO: 1 mg/mL (4.6 mM)
Water: <1 mg/mL
Ethanol: <1 mg/mL
SMILES CodeN[C@H](CC1=CN(C)C2=CC=CC=C12)C(O)=O
SynonymsNLG 8189; 1-Methyl-D-tryptophan; Indoximod; NLG8189; NLG-8189; D1MT;
实验参考方法
In Vitro

In vitro activity: Indoximod significantly reverses the suppression of T cells created by IDO-expressing dendritic cells, using both human monocyte-derived dendritic cells and murine dendritic cells isolated directly from tumor-draining lymph nodes. Indoximod activates a Trp sufficiency signal that stimulates mTOR, and relieves IDO-induced Trp deprivation as well as an autophagic response.


Kinase Assay: 1MT enantiomers are solubilized in DMSO containing 0.1N HCl and added at concentrations of 100, 50, and 0 μM to wells containing the reaction mixture with tryptophan (0-200 μM) followed by addition of IDO enzyme. Plates are sealed and incubated 1 hr in a humidified 37°C incubator, after which the reactions are terminated by addition of 12.5 μl 30% TCA per well. Plates are then resealed in plastic wrap, incubated 30 min at 50°C to hydrolyze the reaction product N-formylkynurenine to kynurenine, and centrifuged. Supernatants are transferred to a flat-bottom 96-well plate, mixed with 100 μl Ehrlich reagent and incubated 10 min at room temperature. Absorbance at 490 nm is read.


Cell Assay: The immunosuppressive activity of IDO leads to an increase in the number of T-regulatory cells, as measured by their Foxp3+/CD4+/CD25+ phenotype. Indoximod has also been shown to reduce the number of T-regulatory cells.

In VivoIn mouse models of transplantable melanoma and transplantable and autochthonous breast cancer, Indoximod (400 mg/kg p.o.) enhances antitumor immunity in the setting of combined chemo-immunotherapy regimens.
Animal modelMouse B16F10 and 4T1 tumor models
Formulation & DosageDissolved in 0.5% Tween 80/0.5% Methylcellulose (v/v) in water; 400 mg/kg; p.o. administration
ReferencesCancer Res. 2007 Jan 15;67(2):792-801; Oncoimmunology. 2012 Dec 1;1(9):1460-1468.