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Liproxstatin-1
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Liproxstatin-1图片
CAS NO:950455-15-9
规格:≥98%
包装与价格:
包装价格(元)
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议
250mg电议
500mg电议

产品介绍
理化性质和储存条件
Molecular Weight (MW)340.85
FormulaC19H21ClN4
CAS No.950455-15-9
Storage-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)DMSO: 68 mg/mL (199.5 mM)
Water: <1 mg/mL
Ethanol: 21 mg/mL (61.6 mM)
SMILES Code ClC1=CC(CNC(C23CCNCC2)=NC4=C(N3)C=CC=C4)=CC=C1
Synonyms

Liproxstatin-1;

Chemical Name: N-[(3-Chlorophenyl)methyl]-spiro[piperidine-4,2′(1′H)-quinoxalin]-3′-amine

实验参考方法
In Vitro

In vitro activity: Liproxstatin-1 is able to inhibit ferroptosis in the low nanomolar range and inhibit the growth of Gpx4–/–cells with IC50 of 22 nM. Liproxstatin-1 (50 nM) completely prevents lipid peroxidation in Gpx4–/–cells. Liproxstatin-1 (200 nM) protects against FINs, such as BSO (10 μM), erastin (1 μM) and RSL3 (0.5 μM), in a dose dependent manner, whereas it fails to rescue cell death induced by staurosporine (0.2 μM) and H2O2 (200 μM).


Kinase Assay: Liproxstatin-1 is a novel, potent inhibitor of ferroptosis with IC50 value of 22 nM in cell free assays.


Cell Assay: Cell viability is assessed at different time points after treatment using AquaBluer according to the manufacturer’s recommendations. Alternatively, cell death is also quantified by measuring released lactate dehydrogenase activity using the cytotoxicity detection kit (LDH).

In VivoLiproxstatin-1 remarkably extends survival compared with the vehicle-treated group, delays ferroptosis in tubular cells, and mitigates tissue injury in ischaemia/reperfusion-induced liver injury.
Animal modelGreERT2; Gpx4fI/fI mice
Formulation & DosageDissolved in % DMSO in PBS; 10 mg/kg; i.p. injection
ReferencesNat Cell Biol. 2014 Dec;16(12):1180-91.