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B02
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
B02图片
CAS NO:1290541-46-6
规格:≥98%
包装与价格:
包装价格(元)
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议
250mg电议
500mg电议

产品介绍
理化性质和储存条件
Molecular Weight (MW)339.39
FormulaC22H17N3O
CAS No.1290541-46-6
Storage-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)DMSO: 67 mg/mL (197.4 mM)
Water: <1 mg/mL
Ethanol: 20 mg/mL (58.9 mM)
SMILES O=C1N(CC2=CC=CC=C2)C(/C=C/C3=CC=CN=C3)=NC4=C1C=CC=C4
Synonyms B02; B-02; B 02; RAD51-IN-02; RAD51-IN 02; RAD51 IN-02; RAD51 Inhibitor B02
实验参考方法
In Vitro

In vitro activity: B02 is a specific inhibitor of human RAD51 recombinase, blocks HR repair in human embryonic kidney (HEK) and breast cancer cells and increases their sensitivity to a wide range of DNA damaging agents. Also, B02 enhances DNA damage and apoptosis induced by decitabine in MM cells. B02 shows high specificity for RAD51 and does not significantly inhibit RAD54 in the range of concentrations from 0 to 200 μM. B02 shows biological effect in human and mouse cells. In human embryonic kidney (HEK) cells, B02 disrupts RAD51 foci formation in response to DNA damage and inhibited DSB repair and DSB-dependent HR. B02 can also increase the sensitivity of cancer cells to chemotherapeutic DNA damaging agents.


Kinase Assay: RAD51 Inhibitor B02 (B02) is an inhibitor of human RAD51 with an IC50 of 27.4 μM.


Cell Assay: Cells are exposed for 1 h, then the cells are ished by PBS three times and refreshed by the media containing B02 (5 μM). After 7-10 days, cells are fixed and stained with staining solution (0.05% crystal violet, 50% methanol in PBS); finally cell colonies are counted.

In VivoB02 significantly increases the anti-tumor activity of cisplatin in vivo. B02 is tolerated by mice at doses up to 50 mg/kg without obvious body weight loss. No detectable morphological changes induced by B02 in kidneys and livers, main organs for detoxification are found.
Animal modelNCR nude mice
Formulation & DosageDissolved in cremophor/DMSO/NS (1:1:3); 50 mg/kg; i.p. injection.
ReferencesFront Oncol. 2014;4:289; ACS Chem Biol. 2011;6(6):628-35; PLoS One. 2014;9(6):e100993.