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Procarbazine HCl(NSC-77213)
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Procarbazine HCl(NSC-77213)图片
CAS NO:366-70-1
规格:≥98%
包装与价格:
包装价格(元)
50mg电议
100mg电议
250mg电议
500mg电议
1g电议
2g电议
5g电议

产品介绍
理化性质和储存条件
Molecular Weight (MW)257.76
FormulaC12H19N3O.HCl
CAS No.366-70-1
Storage-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)DMSO: <1 mg/mL
Water: 52 mg/mL (201.7 mM)
Ethanol: 52 mg/mL (201.7 mM)
Other infoChemical Name: N-isopropyl-4-((2-methylhydrazinyl)methyl)benzamide hydrochloride

InChi Key: DERJYEZSLHIUKF-UHFFFAOYSA-N

InChi Code: InChI=1S/C12H19N3O.ClH/c1-9(2)15-12(16)11-6-4-10(5-7-11)8-14-13-3;/h4-7,9,13-14H,8H2,1-3H3,(H,15,16);1H

SMILES Code: O=C(NC(C)C)C1=CC=C(CNNC)C=C1.[H]Cl

SynonymsNSC-77213 HCl; CB 400-497; NSC-77213; Ro 4-6467; CB 400497; NSC77213; Ro4-6467; CB-400-497; NSC77213; Ro4-6467; Ro 4-6467/1; Procarbazine Hydrochloride; PCB Hydrochloride; PCZ; Procarbazin. Matulane. Natulan; Natulanar; Natunalar.
实验参考方法
In Vitro

In vitro activity: Procarbazine plus Cu(II) induce piperidine-labile and formamidopyrimidine-DNA glycosylase-sensitive lesions at the 5'-ACG-3' sequence, complementary to a hotspot of the p53 gene, and the 5'-TG-3' sequence. Procarbazine causes DNA damage through non-enzymatic formation of the Cu(I)-hydroperoxo complex and methyl radicals. Procarbazine has a strong clastogenic effect in hematopoietic cells and is mutagenic in a variety organs after high dose treatment.


Cell Assay: Following Procarbazine or metabolite treatment, cells are diluted to 50.000/mL in 25-cm2 culture flasks (10 mL). Every 24 h, a 0.5-mL aliquot is removed, diluted 20-fold in Hematall isotonic diluent, and the cell number determined with a Coulter Model F electronic cell counter. Counts greater than 10,000/0.5 mL are corrected for coincidence. Cells are diluted in fresh culture media when cell density exceeded 1 × 106/mL. Cultures are maintained until the aggregate cell number approached 100 × 106/mL and doubling time has returned to 12 h. Cell survival is determined using Equation A, where TD(doubling time for cells of interest) is 12 h.

In VivoProcarbazine causes significant decrease in testicular and epididymal weight and a drastic reduction in haploid cells and spermatogenic arrest, demonstrating variation among the test golden hamster. Procarbazine produces a dose-dependent potentiation of MAO A in brown adipose tissue, the elevation being more pronounced following monomethylhydrazine, with activity rising to 350% of that in control homogenates in rats. Procarbazine or monomethylhydrazine reduces metabolism of this amine by a similar degree as had been determined ex-vivo in blood vessel homogenates. Procarbazine is mutagenic, clastogenic and teratogenic in a wide range of test systems of varying complexity and a wide-spectrum carcinogen in rodents and monkeys, causing tumours of the haemopoietic system, the mammary gland, the lung and the nervous system. Procarbazine in vivo undergoes a complex series of metabolic changes that result in the generation of a number of chemically reactive species, including methylating agents and free radicals.
Animal modelRats
Formulation & Dosage
References

Mutat Res. 2003 Aug 5;539(1-2):145-55; Arch Androl. 2002 Mar-Apr;48(2):91-100.