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Mizoribine(NSC 289637 HE 69)
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Mizoribine(NSC 289637 HE 69)图片
CAS NO:50924-49-7
规格:≥98%
包装与价格:
包装价格(元)
50mg电议
100mg电议
250mg电议
500mg电议
1g电议
2g电议

产品介绍
理化性质和储存条件
Molecular Weight (MW)259.22
FormulaC9H13N3O6
CAS No.50924-49-7
Storage-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)DMSO: <1 mg/mL
Water: 52 mg/mL (200.6 mM)
Ethanol: <1 mg/mL
Solubility (In vivo)

Chemical Name: 1-((2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-hydroxy-1H-imidazole-4-carboxamide

InChi Key: HZQDCMWJEBCWBR-UUOKFMHZSA-N

InChi Code: InChI=1S/C9H13N3O6/c10-7(16)4-8(17)12(2-11-4)9-6(15)5(14)3(1-13)18-9/h2-3,5-6,9,13-15,17H,1H2,(H2,10,16)/t3-,5-,6-,9-/m1/s1

SMILES Code: O=C(C1=C(O)N([C@@H]2O[C@H](CO)[C@@H](O)[C@H]2O)C=N1)N

SynonymsHE 69; He69; NSC289637; NSC-289637; He-69; Mizoribine; Bredinin; NSC 289637.
实验参考方法
In Vitro

In vitro activity: Mizoribine (1-50 mg/mL) is able to inhibit T cell proliferation by 10-100% in a dose-dependent fashion to all stimuli tested. Mizoribine leads to a decrease in intracellular GTP levels, and that repletion of GTP reversed its antiproliferative effects Mizoribine causes a dose-dependent inhibition of T cell proliferation, which is reversible with the addition of 50 mM guanosine to replete guanine ribonucleotide pools. Mizoribine selectively inhibits guanine ribonucleotide formation in purified T cells, whereas the effect of 6-mercaptopurine (6MP) appears to be more dependent on adenine ribonucleotide depletion. Mizoribine inhibits HCV RNA replication with IC50 of 100 μM.


Cell Assay: Unlike azathioprine, Mizoribine is not taken up by nucleic acids in the cell. Instead, after phosphorylation MZR-5 -monophosphate inhibits GMP synthesis by the antagonistic blocking of IMPDH (Ki = 10(-8)M) and GMP- synthetase (Ki =10(-5) M). Pretreatment of cells with MZR partially, but significantly, attenuates the expression of monocyte chemoattractant protein (MCP)-1 mRNA and protein, whereas the poly IC-induced expressions for the other functional molecules, such as CCL5, fractalkine and IL-8 were not influenced by MZR treatment.

In VivoMizoribine (5 or 10 mg/kg) suppresses urinary albumin excretion in the rats. Mizoribine (5 or 10 mg/kg) inhibits the development of glomerulosclerosis, interstitial fibrosis and macrophage infiltration in the kidney in the rats. Mizoribine (5 or 10 mg/kg) increases the expression of MCP-1, OPN and TGF-β1 mRNA in untreated OLETF rats.
Animal modelRats
Formulation & Dosage5 or 10 mg/kg
References

Mol Pharmacol. 1992 Apr;41(4):671-6; Nephrol Dial Transplant. 2005 Aug;20(8):1573-81.