生物活性

Insulin(Pig) 猪胰岛素是机体内唯一降低血糖的蛋白激素，不仅负责调控细胞对葡萄糖、氨基酸和脂肪酸的摄入，利用和储存，也是唯一同时促进糖原、脂肪、蛋白质合成的激素。

*该产品在溶液状态不稳定，建议您现配现用，尽快使用
本产品溶液不能进行高压灭菌。
本品也可溶于125mM NaHCO3。不推荐配制碱性储存液，因为高pH值会提高脱酰胺反应和蛋白聚集。

General recommendations:
1. Do not prepare concentrated insulin solutions, as solubility is difficult and multimers can form.
2. Adjust to acidic pH to facilitate solubilization and minimize the effects of neutral pH-binding proteases. For added protection, consider adding an acid protease inhibitor.
3. Sterile-filter the solution using a 0.2 micron low protein-binding filter. Avoid aeration and/or foaming during solubilization and filtration, as this can cause protein denaturation.
4. Store solutions at 2-8 °C for up to 8 hours.
5. Do not heat above 30 °C and do not freeze.
6. The recommended insulin working concentration is 1-10 μg/mL.

As an alternative, Insulin solutions can be prepared with phosphate buffered solution (PBS). Resolubilize the aforementioned products in PBS at a concentration of 1 mg/mL. Allow to gently rock for at least 1-2 hours at room temperature. Do not vortex. Solution is ready when clear. Prior to evaluation, follow steps 3–6 for final processing.

化学数据

不同实验动物依据体表面积的等效剂量转换表（数据来源于FDA指南）

例如，依据体表面积折算法，将化合物用于小鼠的剂量20 mg/kg 换算成大鼠的剂量，需要将20 mg/kg 乘以小鼠的K_m系数（3），再除以大鼠的K_m系数（6），得到化合物用于大鼠的等效剂量为10 mg/kg。

储备液配制

以下数据基于产品分子量，对于特殊产品，请参照COA中的储备液配制条件和说明进行操作。