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Colchicine
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Colchicine图片
CAS NO:64-86-8
包装:100mg, 500mg
包装与价格:
包装价格(元)
Free Sample (0.5-1 mg)电议
10mM*1mL in DMSO电议
100mg电议
500mg电议

产品介绍

生物活性

Colchicine是一种微管蛋白抑制剂和微管干扰剂,其IC50值为3 nM。Colchicine 还是 α3 甘氨酸受体 (GlyRs) 的竞争性拮抗剂。Colchicine is a toxic natural product and secondary metabolite, originally extracted from plants of the genus Colchicum. Colchicine inhibited stimulated endothelial adhesiveness via its effects on microtubules since vinblastine, an agent which perturbs microtubule function by other mechanisms, diminished adhesiveness whereas the photoinactivated colchicine derivative gamma-lumicolchicine was inactive. Colchicine (Colchineos, Colchisol, Colcin) had no effect on cell viability. At higher, therapeutic, concentrations colchicine (IC50 = 300 nM, P < 0.001) also diminished the expression of L-selectin on the surface of neutrophils (but not lymphocytes) without affecting expression of the beta 2-integrin CD11b/CD18. Since one of the defining characteristics of cancer cells is a significantly increased rate of mitosis, this means that cancer cells are significantly more vulnerable to colchicine poisoning than are normal cells. However, the therapeutic value of colchicine against cancer is (as is typical with chemotherapy agents) limited by its toxicity against normal cells.


化学数据

分子量399.44
分子式C22H25NO6
CAS号64-86-8
纯度>99%
溶解性(25°C)DMSO 45 mg/mL
Water 30 mg/mL
储存和运输条件固体粉末: -20°C 冷藏长期储存
常温运输及临时存放

实验操作 来自于公开的文献,仅供相同实验参考(如实验材料、目的不同,请参考其他文献)

细胞实验
细胞系COS-7 cells
方法After 20 min, the samples were mixed with 5 nM CT20126, colchicine or Taxol and incubated at 37 °C for 5 min. The samples were cooled in an ice bath for depolymerization (still within the light path cell). Successive reactions were performed for another 25 min at 37 °C.
浓度5 nM
处理时间5 min

动物实验
动物模型Male Sprague–Dawley rats
配制water
剂量5 mg/kg
给药处理gavage

不同实验动物依据体表面积的等效剂量转换表(数据来源于FDA指南)

小鼠大鼠豚鼠仓鼠
重量 (kg)0.020.151.80.40.0810
体表面积 (m2)0.0070.0250.150.050.020.5
Km系数36128520
动物 A (mg/kg) = 动物 B (mg/kg) × 动物 B的Km系数
动物 A的Km系数

例如,依据体表面积折算法,将化合物用于小鼠的剂量20 mg/kg 换算成大鼠的剂量,需要将20 mg/kg 乘以小鼠的Km系数(3),再除以大鼠的Km系数(6),得到化合物用于大鼠的等效剂量为10 mg/kg。


储备液配制

以下数据基于产品分子量,对于特殊产品,请参照COA中的储备液配制条件和说明进行操作。

Concentration / Solvent Volume / Mass1 mg5 mg10 mg
1 mM2.5035 mL12.5175 mL25.035 mL
5 mM0.5007 mL2.5035 mL5.007 mL
10 mM0.2504 mL1.2518 mL2.5035 mL