生物活性
Podophyllotoxin (Podofilox) is a lignan (lignans) found in podophyllin resin from the roots of podophyllum plants. It is a potent spindle poison, toxic if taken internally, and has been used as a cathartic. It is very irritating to skin and mucous membranes, has keratolytic actions, has been used to treat warts and keratoses, and may have antineoplastic properties, as do some of its congeners and derivatives.
化学数据
| 分子量 | 414.41 |
| 分子式 | C22H22O8 |
| CAS号 | 518-28-5 |
| 纯度 | 96.63% |
| 溶解性（25°C） | DMSO 63 mg/mL |
| 储存和运输条件 | 固体粉末： -20°C 冷藏长期储存
常温运输及临时存放 |
实验操作 来自于公开的文献，仅供相同实验参考（如实验材料、目的不同，请参考其他文献）
| 细胞实验 |
|---|
| 细胞系 | Human glioma cell lines CL5 |
| 方法 | After the Etoposide treatment, removing cells from the dish with phosphate-buffered saline (PBS) containing 0.03% trypsin and 0.27 mM ethylenediaminetetraacetic acid (EDTA) and diluting into culture dishes in appropriate numbers to yield between 20 and 200 colonies. 12 days later, fixing cultures with methanol-acetic acid, staining with crystal violet, and scoring for colonies containing more than 50 cells. The standard errors are typically less than 15% of the mean value unless otherwise stated. |
| 浓度 | 80 μg/mL |
| 处理时间 | 1 hour |
| 动物实验 |
|---|
| 动物模型 | Murine angiosarcoma xenografts ISOS-1 |
| 配制 | Saline |
| 剂量 | 10 mg/kg |
| 给药处理 | i.p. every day for 5 days from day 7 |
不同实验动物依据体表面积的等效剂量转换表（数据来源于FDA指南）
| 小鼠 | 大鼠 | 兔 | 豚鼠 | 仓鼠 | 狗 |
| 重量 (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| 体表面积 (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km系数 | 3 | 6 | 12 | 8 | 5 | 20 |
| 动物 A (mg/kg) = 动物 B (mg/kg) × | 动物 B的Km系数 |
| 动物 A的Km系数 |
例如，依据体表面积折算法，将化合物用于小鼠的剂量20 mg/kg 换算成大鼠的剂量，需要将20 mg/kg 乘以小鼠的Km系数（3），再除以大鼠的Km系数（6），得到化合物用于大鼠的等效剂量为10 mg/kg。
储备液配制
以下数据基于产品分子量，对于特殊产品，请参照COA中的储备液配制条件和说明进行操作。
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.4131 mL | 12.0653 mL | 24.1307 mL |
| 5 mM | 0.4826 mL | 2.4131 mL | 4.8261 mL |
| 10 mM | 0.2413 mL | 1.2065 mL | 2.4131 mL |
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