生物活性
Cyclophosphamide是一种氮芥类烷化剂，使烷基连接到DNA的鸟嘌呤碱基。Cyclophosphamide inhibits the AChE reversibly with an IC50 of 511 microM. The Michaelis-Menten constant (Km) was 132 microM for AChE in the control system; a value increased by 78% in the CP treated system. The Vmax was 73.8 mumol/h/mg protein for the control system. Cyclophosphamide is used to treat cancers and autoimmune disorders. Cyclophosphamide belongs to a group of drugs called alkylating agents. It works by sticking to one of the cancer cell's DNA strands. DNA is the genetic code that is in the heart of all animal and plant cells. It controls everything the cell does.The cell cannot then divide into 2 new cells.
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化学数据
| 分子量 | 261.09 |
| 分子式 | C7H15Cl2N2O2P |
| CAS号 | 50-18-0 |
| 纯度 | >98% |
| 溶解性（25°C） | DMSO 35 mg/mL
Water 30 mg/mL |
| 储存和运输条件 | 2-8°C
常温运输及临时存放 |
实验操作 来自于公开的文献，仅供相同实验参考（如实验材料、目的不同，请参考其他文献）
| 细胞实验 |
|---|
| 细胞系 | CD4+ T-cell |
| 方法 | Total CD4+ T cells, labeled with αCD25 BV605, were cultured for 3 days in RPMI/10% AB containing 0 or 50 IU/mL IL-2 as indicated, in presence of CPA (0 μM-1000 μM), mafosfamide (MAF; stabilized active derivative of CPA) (0 μM, 1.2 μM, 12 μM), and/or verapamil (10 μM). Doses of CPA and MAF were titrated to identify doses discriminating sensitivity of Treg and non-Treg cell subsets. |
| 浓度 | 0 μM, 1.2 μM, 12 μM |
| 处理时间 | 3 d |
| 动物实验 |
|---|
| 动物模型 | Sprague–Dawley rats |
| 配制 | |
| 剂量 | 25mg/kg |
| 给药处理 | i.v. |
不同实验动物依据体表面积的等效剂量转换表（数据来源于FDA指南）
| 小鼠 | 大鼠 | 兔 | 豚鼠 | 仓鼠 | 狗 |
| 重量 (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| 体表面积 (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km系数 | 3 | 6 | 12 | 8 | 5 | 20 |
| 动物 A (mg/kg) = 动物 B (mg/kg) × | 动物 B的Km系数 |
| 动物 A的Km系数 |
例如，依据体表面积折算法，将化合物用于小鼠的剂量20 mg/kg 换算成大鼠的剂量，需要将20 mg/kg 乘以小鼠的Km系数（3），再除以大鼠的Km系数（6），得到化合物用于大鼠的等效剂量为10 mg/kg。
储备液配制
以下数据基于产品分子量，对于特殊产品，请参照COA中的储备液配制条件和说明进行操作。
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 3.8301 mL | 19.1505 mL | 38.301 mL |
| 5 mM | 0.766 mL | 3.8301 mL | 7.6602 mL |
| 10 mM | 0.383 mL | 1.915 mL | 3.8301 mL |
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