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Resiquimod(R848)
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Resiquimod(R848)图片
CAS NO:144875-48-9
规格:≥98%
包装与价格:
包装价格(元)
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议
250mg电议
500mg电议
1g电议

产品介绍
理化性质和储存条件
Molecular Weight (MW)314.38
FormulaC17H22N4O2
CAS No.144875-48-9
Storage-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)DMSO: 63 mg/mL (200.4 mM)
Water: <1 mg/mL
Ethanol: 21 mg/mL (66.8 mM)
SMILES CodeCC(O)(C)CN1C(COCC)=NC2=C1C3=CC=CC=C3N=C2N
SynonymsR-848; S-28463; VML600; R848; S 28463; VML 600; R 848; S28463; VML-600.
实验参考方法
In Vitro

In vitro activity: Resiquimod activates immune cells and induces proliferation of wild-type splenocytes via the Toll-like receptor 7 (TLR7)-MyD88-dependent signaling pathway. Resiquimod also modulates dendritic cells to augment cytomegalovirus- and HIV-1-specific T cell responses. Resiquimod induces the differentiation of myeloid-derived suppressor cells into macrophages and dendritic cells, and may improve cancer immunotherapy by reducing immunosuppressive MDSCs.


Kinase Assay: For luciferase assay, FG-9307 cells are transfected with the firefly NF-κB-specific luciferase reporter vector pNFκB-Met-Luc2. Transfection efficiency is monitored by co-transfection with the pSEAP2 control vector, which constitutively expresses the human secreted enhanced alkaline phosphatase (SEAP). Then the cells are treated with Resiquimod (R848, 1 μg/mL), CQ (10 μM), CQ plus R848 or PBS and incubated at 22°C for 24 h. The culture medium of the transfectants is then analyzed for luciferase activity and SEAP activity using Luciferase Assay Kit and the Great EscAPe(TM) SEAP Chemiluminescence Detection Kit, respectively. The assay is performed three times.


Cell Assay: Unlike LPS-induced activation, which was MyD88-independent, Resiquimod-induced activation of NF-κB was completely dependent on MyD88. It was implied that Resiquimod activated macrophages via TLRs.

In VivoIn wild-type mice, Resiquimod (50 nmol, i.p.) induces increased serum concentrations of IFN-alpha, TNF-alpha and IL-12, while neither TLR7-deficient mice nor MyD88-deficient mice show an increase in these cytokines. In a murine model of allergic asthma, Resiquimod (i.n., 20 μg/mouse) reduces allergen induced airway reactivity and inflammation via reduction in Nrf2 signaling.
Animal modelWild-type mice, TLR7-deficient mice, and MyD88-deficient mice
Formulation & DosageDissolved in saline; 50 nmol; i.p. injection
ReferencesNat Immunol. 2002 Feb;3(2):196-200; J Immunol. 2003 Oct 15;171(8):4320-8.