您好,欢迎来到化工原料网! [登录] [免费注册]
化工原料网
位置:首页 > 产品库 > Crosstide
立即咨询
咨询类型:
     
*姓名:
*电话:
*单位:
Email:
*留言内容:
请详细说明您的需求。
*验证码:
 
Crosstide
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
CAS NO:171783-05-4
规格:98%
分子量:1164.23
包装与价格:
包装价格(元)
1mg电议
5mg电议

产品介绍
Crosstide是一种糖原合成酶激酶α/β融合蛋白序列的模拟肽,是Akt的底物。
CAS:171783-05-4
分子式:C48H77N17O17
分子量:1164.23
纯度:98%
存储:Store at -20°C

Background:

Crosstide is a peptide analog of glycogen synthase kinase α/β fusion protein sequence which is a substrate for Akt.


16HBE14o- cells exposed to RV39 demonstrate a crosstide kinase activity in vitro. Serine phosphorylation of crosstide is confirmed by immunoblotting, and phosphorylation is blocked by PP2 but not by PP3[1]. The wildtype GST-AKT2 shows significant phosphoryl transferase activity towards crosstide, reaching an initial velocity of 16 pmol phosphate/min/μg enzyme. The mutant GST-AKT2T/E,S/D displayed an initial velocity of 85 pmol phosphate/min/μg kinase for phosphorylation of Crosstide, corresponding to a 5-fold increase compared to the wildtype enzyme[2].



[1]. Bentley JK, et al. Rhinovirus activates interleukin-8 expression via a Src/p110beta phosphatidylinositol 3-kinase/Akt pathway in human airway epithelial cells. J Virol. 2007 Feb;81(3):1186-94. Epub 2006 Nov 22. [2]. Baer K, et al. Activation of a GST-tagged AKT2/PKBbeta. Biochim Biophys Acta. 2005 Oct 10;1725(3):340-7. Epub 2005 Apr 20.