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Coelenterazine
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Coelenterazine图片
CAS NO:55779-48-1
规格:98%
分子量:423.46
包装与价格:
包装价格(元)
1mg电议
5mg电议

产品介绍
luminescent enzyme substrate
CAS:55779-48-1
分子式:C26H21N3O3
分子量:423.46
纯度:98%
存储:Store at -20°C

Background:

Target: superoxide anion


IC50: 69 μM (rat hepatocytes cells)


The small molecule coelenterazine, a luminescent enzyme substrate, can react with the reactive oxygen species (ROS) superoxide anion and peroxynitrite and produce a chemiluminescent signal [1]. Therefore, coelenterazine is used for monitoring reporter genes in ELISA, BRET, and HTS techniques. Reactive oxygen species (ROS) play an important role in tumor development and survival and it is a new treatment strategy to target the cancer-specific ROS.


In vitro: Coelenterazine showed antioxidative properties in primary cultures of rat hepatocytes subjected to the oxidant tert-butyl hydroperoxide (t-BHP). Coelenterazine (3-10 μM) concentration-dependently increased survival and decreased lipid peroxidation in rat hepatocytes treated for 6 hr with t-BHP (250 μM) [2]. In addition, Coelenterazine (10 μmol/l) can be used to assay superoxide anion concentrations in cancer cell lines [1].


In vivo: the small molecule coelenterazine (5 mg/kg, intravenous administration) was a potential reporter of cancer-associated superoxide anion in the 4T1 breast adenocarcinoma mouse model [1]. Gli36-Rluc or Gli36-Gluc cells were injected into the leg muscle of nude mice. The mice were injected with 4 mg/kg of coelenterazine and imaged by a cooled charge-coupled device camera [3].


参考文献:
1. Bronsart LL, Stokes C, Contag CH. Multimodality Imaging of Cancer Superoxide Anion Using the Small Molecule Coelenterazine. Mol Imaging Biol. 2016;18(2):166-71.
2. Dubuisson ML, de Wergifosse B, Trouet A, Baguet F, Marchand-Brynaert J, Rees JF. Antioxidative properties of natural coelenterazine and synthetic methyl coelenterazine in rat hepatocytes subjected to tert-butyl hydroperoxide-induced oxidative stress. Biochem Pharmacol. 2000;60(4):471-8.
3. Morse D, Tannous BA. A water-soluble coelenterazine for sensitive in vivo imaging of coelenterate luciferases. Mol Ther. 2012;20(4):692-3.