PNPP是用于ELISA和常规分光光度测定的碱性和酸性磷酸酶的非蛋白质性显色底物。
CAS:4264-83-9
分子式:C6H4NNa2O6P
分子量:263.05
纯度:98%
存储:Store at -20°C
Background:
PNPP is a non-proteinaceous chromogenic substrate for alkaline and acid phosphatases used in ELISA and conventional spectrophotometric assays.
Phosphatases catalyze the hydrolysis of PNPP liberating inorganic phosphate and the conjugate base of para-nitrophenol (pNP). The resulting phenolate is yellow, with a maximal absorption at 405 nm[1].