Background:

Neoruscogenin is a bioavailable, potent, and high-affinity agonist of the nuclear receptor RORα [1]. Neoruscogenin is a natural sapogenin extracted from Butcher’s broom (Rusci rhizoma, Ruscus aculeatus, Ruscaceae) [2].

RAR-related orphan receptor alpha (RORα) is a nuclear receptor that participates in the transcriptional regulation of some genes involved incircadian rhythm. Genetic studies have revealed that RORα plays an important role in the development of the central nervous system and has been associated with genetic lesions in mice [3].

In vitro: In confluent human microvascular endothelial cells (HMEC-1), pre-treatment with neoruscogenin slightly decreased the permeability at concentrations up to 100 μM (71.8%). The EC50 of neoruscogenin against the nuclear receptor RORα was 110 nM [1].

In vivo: In mice, oral administration of neoruscogenin (3 mg/kg/d) for seven days up-regulated the expression of several RORα-inducible genes in the liver [1].

参考文献:
[1] Helleboid S, Haug C, Lamottke K, et al.  The identification of naturally occurring neoruscogenin as a bioavailable, potent, and high-affinity agonist of the nuclear receptor RORα (NR1F1)[J]. Journal of biomolecular screening, 2013: 1087057113497095.
[2] Barbi M, Willer E A, Rothenhfer M, et al.  Spirostanol saponins and esculin from Rusci rhizoma reduce the thrombin-induced hyperpermeability of endothelial cells[J]. Phytochemistry, 2013, 90: 106-113.
[3] Giguère V.  Orphan nuclear receptors: from gene to function 1[J]. Endocrine reviews, 1999, 20(5): 689-725.