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ONPG(2-Nitrophenylβ-D-galactopyranoside)
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
ONPG(2-Nitrophenylβ-D-galactopyranoside)图片
CAS NO:369-07-3
规格:98%
分子量:301.25
包装与价格:
包装价格(元)
1g电议
5g电议

产品介绍
ONPG是一种比色和分光光度计底物,用于检测β-半乳糖苷酶活性。
CAS:369-07-3
分子式:C12H15NO8
分子量:301.25
纯度:98%
存储:Store at -20°C

Background:

ONPG is a colorimetric and spectrophotometric substrate for detection of β-galactosidase activity.


The enzyme displays high hydrolysis ability for ONPG (100%) and moderate activity for its natural substrate lactose (25.7%). However, the hydrolysis ability of the enzyme towards all other chromogenic nitrophenyl analogues is very weak, indicating that Gal308 is a β-galactosidase with narrow substrate specificity. To investigate the kinetic parameters of recombinant enzyme, the Michaelis-Menten constants (Km), turnover numbers (kcat), and catalytic efficiencies (kcat/Km) of Gal308 for ONPG and lactose are determined. The kcat and Km values are 464.7±7.8 s-1 and 2.7±0.3 mM for ONPG, and 264.2±2.1 s-1 and 7.1±0.8 mM for lactose, respectively. The kcat/Km value of the enzyme for ONPG (172.1 s-1mM-1) is 4.6-fold higher than that for lactose (37.2 s-1mM-1), which clearly demonstrated that the catalytic efficiency of Gal308 for ONPG is much higher than that for lactose[1].



[1]. Zhang X, et al. Metagenomic approach for the isolation of a thermostable β-galactosidase with high tolerance of galactose and glucose from soil samples of Turpan Basin. BMC Microbiol. 2013 Oct 24;13:237. doi: 10.1186/1471-2180-13-237.