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DAF 2DA(DAF-2DA)
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
DAF 2DA(DAF-2DA)图片
CAS NO:205391-02-2
规格:98%
分子量:446.41
包装与价格:
包装价格(元)
1mg电议
5mg电议

产品介绍
DAF2DA是测量NO最广泛使用的一种荧光探针。
CAS:205391-02-2
分子式:C24H18N2O7
分子量:446.41
纯度:98%
存储:Store at -20°C

Background:

DAF 2DA is most widely probe for NO measurement.


Nitric oxide is a free radical signal molecule. Various methods are available for measurement of NO. Out of all methods, fluorescent probes to localize NO is very widely used method. Diaminofl uorescein in diacetate form (DAF 2DA) is most widely probe for NO measurement. This method is based on application of 4,5-diaminofl uorescein diacetate (DAF 2DA) which is actively diffused into cells, once taken up by cells cytoplasmic esterases cleave the acetate groups to generate 4,5-diaminofl uorescein; DAF-2. The generated DAF-2 can readily react with N2O3, which is an oxidation product of NO to generate the highly fluorescent DAF-2T (triazolofl uorescein).



[1]. Wany A, et al. Localization of Nitric Oxide in Wheat Roots by DAF Fluorescence. Methods Mol Biol. 2016;1424:39-47.