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OAC1(BAS 00287861)
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
OAC1(BAS 00287861)图片
CAS NO:300586-90-7
规格:≥98%
包装与价格:
包装价格(元)
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议
250mg电议
500mg电议

产品介绍
理化性质和储存条件
Molecular Weight (MW)237.26
FormulaC14H11N3O
CAS No.300586-90-7
Storage-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)DMSO: 47 mg/mL (198.1 mM)
Water: <1 mg/mL
Ethanol: 21 mg/mL (88.5 mM)
Other info

Chemical Name: N-(1H-pyrrolo[2,3-c]pyridin-5-yl)benzamide

InChi Key: HWJRIFZDXJKJJN-UHFFFAOYSA-N

InChi Code: InChI=1S/C14H11N3O/c18-14(10-4-2-1-3-5-10)17-13-8-11-6-7-15-12(11)9-16-13/h1-9,15H,(H,16,17,18)

SMILES Code: O=C(NC1=CC2=C(NC=C2)C=N1)C3=CC=CC=C3

SynonymsBAS-00287861; BAS00287861; BAS 00287861; OAC 1; OAC1; OAC-1
实验参考方法
In Vitro

In vitro activity: OAC1 at 1 μM enhances reprogramming efficiency by activating both Oct4 and Nanog promoter-driven luciferase reporter genes. Furthermore, OAC1 enhances the pluripotent stem cells (iPSC) reprogramming efficiency and accelerates the reprogramming process in the quartet reprogramming factors (Oct4, Sox2, c-Myc, and Klf4) treated mouse embryonic fibroblasts (MEFs). The iPSC colonies derived using OAC1 along with the quartet factors exhibits typical ESC morphology, gene-expression pattern, and developmental potential. OAC1 seems to enhance reprogramming efficiency via increasing transcription of the Oct4-Nanog-Sox2 triad and Tet1, a gene known to be involved in DNA demethylation. While it doesn’t inhibit the p53-p21 pathway or activate the Wnt-β-catenin signaling. OAC1 may be used to enhance the reprogramming of somatic cells to a pluripotent state.


Cell Assay: The Oct4-luc or Nanog-luc cells are treated with compound OAC1 at 1 μM concentration. Luciferase reporter assays are performed 24 h after OAC1 treatment.

In VivoIn mouse embryonic fibroblasts (MEFs) with the 4F reprogramming quartet Oct4, Klf4, Sox2, and c-Myc, OAC1 enhanced reprogramming efficiency and increased the number of colonies with ESC-like morphology by twofold or more. Also, OAC1 increased induced pluripotent stem cells (iPSC)-like colonies. OAC1 had no effects on the Wnt signaling and p53-p21 expression. In MEFs, OAC1 activated the expression of endogenous Oct4 and Nanog. Also, OAC1 increased the expression of Sox2 and Tet1.
Animal model
Formulation & Dosage
References

Proc Natl Acad Sci U S A. 2012 Dec 18;109(51):20853-8.