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Ac-WEHD-AFC
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Ac-WEHD-AFC图片
CAS NO:210344-99-3
规格:98%
分子量:838.74
包装与价格:
包装价格(元)
1mg电议
5mg电议
10mg电议

产品介绍

Ac-WEHD-AFC 是一种荧光 caspase-1 底物。Ac-WEHD-AFC 可检测 caspase-1 荧光活性,用于肿瘤和炎症的研究。


CAS:210344-99-3
分子式:C38H37F3N8O11
分子量:838.74
纯度:98%
存储:Store at -20°C

Background:

In vitro:Caspase-1 Fluorogenic Activity Assay:[1]

1.Incubate the cells according to your normal protocol. 

2. Cells are transiently transfected with caspase-1 in combination with pcDNA3.1, Myc-cIAP2, Myc-cIAP1, or Myc-XIAP and collected 24 hr later. 

3. Cells were lysed in CHEGG buffer before sonicating for 15 s at 60% amplitude (for cleavage assays). 

4. Lysates were incubated with 10 mM of the fluorogenic caspase-1 substrate Ac-WEHD-AFC. 

5. The release of free AFC was monitored continuously for 1 hr (excitation 380 nm, emission 460 nm) in 1 min intervals and expressed as arbitrary fluorescence units per minute.

参考文献:

[1]. Katherine Labbé, et al. Cellular inhibitors of apoptosis proteins cIAP1 and cIAP2 are required for efficient caspase-1 activation by the inflammasome. Immunity