CAS NO: | 210344-99-3 |
规格: | 98% |
分子量: | 838.74 |
包装 | 价格(元) |
1mg | 电议 |
5mg | 电议 |
10mg | 电议 |
Ac-WEHD-AFC 是一种荧光 caspase-1 底物。Ac-WEHD-AFC 可检测 caspase-1 荧光活性,用于肿瘤和炎症的研究。
CAS:210344-99-3
分子式:C38H37F3N8O11
分子量:838.74
纯度:98%
存储:Store at -20°C
Background:
In vitro:Caspase-1 Fluorogenic Activity Assay:[1]
1.Incubate the cells according to your normal protocol.
2. Cells are transiently transfected with caspase-1 in combination with pcDNA3.1, Myc-cIAP2, Myc-cIAP1, or Myc-XIAP and collected 24 hr later.
3. Cells were lysed in CHEGG buffer before sonicating for 15 s at 60% amplitude (for cleavage assays).
4. Lysates were incubated with 10 mM of the fluorogenic caspase-1 substrate Ac-WEHD-AFC.
5. The release of free AFC was monitored continuously for 1 hr (excitation 380 nm, emission 460 nm) in 1 min intervals and expressed as arbitrary fluorescence units per minute.
参考文献:
[1]. Katherine Labbé, et al. Cellular inhibitors of apoptosis proteins cIAP1 and cIAP2 are required for efficient caspase-1 activation by the inflammasome. Immunity