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SUN-11602
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
SUN-11602图片
CAS NO:704869-38-5
规格:≥98%
包装与价格:
包装价格(元)
25mg电议
50mg电议
100mg电议

产品介绍
理化性质和储存条件
Molecular Formula: C26H37N5O2;
Molecular Weight: 451.615

In Vitro

In vitro activity: SUN11602, a novel synthetic aniline compound with basic fibroblast growth factor-like activity, can mimic the neuroprotective mechanisms of basic fibroblast growth factor. Basic fibroblast growth factor (FGF-2/bFGF) possesses neuroprotective activity and promotes cell proliferation. SUN11602 exhibited neuroprotective activities similar to those of FGF-2 without promoting cell proliferation. In primary cultures of hippocampal neurons, stimulation with SUN11602 or FGF-2 increased calbindin D-28k (CalB) gene expression and prevented glutamate-induced neuronal death. These effects were abolished by pretreatment with PD166866 (FGF receptor 1 [FGFR1] tyrosine kinase-specific inhibitor). This indicated that FGFR1 activation and increased CalB expression were involved in SUN11602-mediated neuroprotection. However, receptor-binding assays revealed that unlike FGF-2, SUN11602 did not alter the binding of (125)I-labeled FGF-2 to FGFR1.


Kinase Assay: SUN11602 is a novel aniline compound with basic fibroblast growth factor-like activity.


Cell Assay: Cerebrocortical neurons are pretreated with vehicle (Hanks’ Balanced Salt Solution), SUN11602, bFGF, or the other growth factors for 24 h prior to the onset of glutamate toxicity. Subsequently, 10 μL of the MTT solution (5 mg/mL) is added to each well (200 μL of culture medium) of the microplates. Neurons in each well are then dried for 24 h, and 200 μL of DMSO is poured into all of the wells in order to dissolve the reaction products thoroughly for the MTT assay.

In VivoIn in vivo studies, wild-type (WT) and CalB-deficient (CalB(-/-)) mice were injected with aggregated Aβ1-40 and ibotenate (NMDA receptor agonist) to severely damage the hippocampal tissue. Treatment with SUN11602 (orally) or FGF-2 (intraparenchymally) at the midpoint of Aβ1-40 and ibotenate injections prevented the hippocampal damage in WT mice, however this effect was abolished in CalB(-/-) mice. Thus, SUN11602 exerted protective effects on hippocampal neurons through activation of FGFR1 and increased CalB expression. Moreover, the neuroprotective effects of SUN11602 depended upon the various biological activities of FGF-2.
Animal modelRats with hippocampal-lesion model
Formulation & Dosage0.1,1,and10mg/kg; orally
ReferencesACS Chem Neurosci. 2013 Feb 20;4(2):266-76; Brain Res. 2015 Jan 12;1594:71-81.