Yellow dye (Reactive group: free acid). Applications: super resolution microscopy e.g. dSTORM & STED, confocal, flow and live cell imaging
货号:ajcx27640
CAS:2245946-45-4
分子式:C27H22N2O5.C2HF3O2
分子量：568.5
溶解度:Soluble in DMSO
纯度：98%
存储：Store at -20°C
库存：现货

Background:

Yellow fluorescent dye; supplied as a free acid. Used in protocol (2017 Grimm et al - see references below) for the synthesis of Janelia Fluor® HaloTag® and SNAP-Tag® ligands, for use in live cell imaging experiments. Also suitable for flow cytometry. Excitation maximum = 549 nm; emission maximum = 571 nm; Quantum yield = 0.88; Extinction coefficient = 101,000 M-1cm-1.

Grimm et al (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat. Methods 12 244 PMID:25599551 |Grimm et al (2017) Synthesis of Janelia Fluor HaloTag and SNAP-Tag Ligands and Their Use in Cellular Imaging Experiments. Methods Mol.Biol. 1663 179 PMID:28924668