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Cy5 alkyne(non-sulfonated)
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Cy5 alkyne(non-sulfonated)图片
规格:98%
分子量:556.18
包装与价格:
包装价格(元)
25mg电议
5mg电议

产品介绍
used for Click chemistry to label any molecule bearing azide group.
货号:ajcx11310
CAS:N/A
分子式:C35H42ClN3O
分子量:556.18
溶解度:≥ 55.6mg/mL in DMSO, ≥ 50 mg/mL in EtOH with ultrasonic
纯度:98%
存储:Store at -20°C
库存:现货

Background:

Cy5 alkyne, with low aqueous solubility is a dye ready for the use in Click Chemistry reaction. Various molecules via Click Chemistry reaction can be attached by this deeply colored, and photostable Cy5 fluorophore. Most derivatives of non-sulfonated cyanines have low aqueous solubility except for hydrochlorides of hydrazides and amines. For biomolecule labeling, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer. Various substrates such as biomolecules, polymers, and solid surfaces which bearing azides can be used for the labeling.

In the azido-GalNAc samples, alkyne-Cy3 and alkyne-Cy5 specifically label azido-proteins. Furthermore, researches also indicate that the Cy5 and Cy3 probe-protein conjugates have non-overlapping fluorescence profiles and can be differentially detected within a single gel [1].

参考文献:
[1] Burnham-Marusich, A. R.; Plechaty, A.M.; Berninsone, P.M. Size-matched alkyne-conjugated cyanine fluorophores to identify differences in protein glycosylation. Electrophoresis, 2014, 35(18), 2621-2625.