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WST-8
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
WST-8图片
规格:98%
分子量:600.47
包装与价格:
包装价格(元)
25mg电议
50mg电议
100mg电议
200mg电议

产品介绍
WST-8 是一种水溶性四唑鎓染料,与传统的基于 MTS 的测定相比,WST-8 提高了基于 WST-8 的测定的灵敏度。
货号:ajcx11028
CAS:193149-74-5
分子式:C20H14N6NaO11S2+
分子量:600.47
溶解度:DMSO: 1 mg/ml,PBS (pH 7.2): 5 mg/ml
纯度:98%
存储:Store at -20°C
库存:现货

Background:

WST-8 is a water-soluble tetrazolium dye, WST-8 enhances sensitivity of the WST-8-based assay over the conventional MTS-based assay.

The generally used MTS-based assay is compared with a bioassay employing a water-soluble tetrazolium dye, WST-8, using NFS-60 cells at a concentration of 7×105 cells/mL against 800 IU/mL of PEGylated G-CSF at 24, 48, 72, and 72 h time points to determine the efficacy of PEGylated G-CSF. Further, the optimized WST-8 dye-based assay is used to test the potency of various commercially available PEGylated G-CSF preparations. The results demonstrate enhanced sensitivity of the WST-8-based assay over the conventional MTS-based assay for determining the potency of PEGylated G-CSF using the NFS-60 cell line[1].

参考文献:
[1]. Tiwari K, et al. A sensitive WST-8-based bioassay for PEGylated granulocyte colony stimulating factor using the NFS-60 cell line. Pharm Biol. 2015 Jun;53(6):849-54.