A fluorogenic substrate for caspase-8
货号:ajcx26380
CAS:N/A
分子式:C31H38F3N5O12 • XCF3COOH
分子量：729.7
溶解度:DMSO: 10 mg/ml
纯度：98%
存储：Store at -20°C
库存：现货

Background:

Ac-IETD-AFC is a fluorogenic substrate that can be cleaved by caspase-8 and related enzymes that recognize the amino acid sequence IETD, including caspase-3 processing enzyme, caspase-10, and granzyme B.¹Caspase activity can be quantified by fluorescent detection of free AFC (also known as 7-amino-4-trifluoromethylcoumarin), which is excited at 400 nm and emits at 505 nm.

1.Reszka, P., Schulz, R., Methling, K., et al.Synthesis, enzymatic evaluation, and docking studies of fluorogenic caspase 8 tetrapeptide substratesChemMedChem5(1)103-117(2010)