A chromogenic and electrochemical substrate for alkaline phosphatase
货号:ajcx25220
CAS:3318-43-2
分子式:C8H6NO4P·2Na
分子量：257.1
溶解度:PBS (pH 7.2): 10 mg/ml
纯度：98%
存储：Store at -20°C
库存：现货

Background:

3-Indoxyl phosphate is a chromogenic and electrochemical substrate for alkaline phosphatase.^1,2Upon enzymatic cleavage by alkaline phosphatase, 3-indoxyl is released and subsequently oxidized to produce water-insoluble indigo, which can be quantified by colorimetric detection at 660 nm as a measure of alkaline phosphatase activity.¹3-Indoxyl phosphate can also be converted to water-soluble leucoindigo by reduction of indigo in alkaline solution by sodium dithionite, which can be quantified by colorimetric detection at 415 nm or voltametric detection as a measure of alkaline phosphatase activity.²3-Indoxyl phosphate has been used in ELISAs.

1.Tsou, K.C., and Su, H.C.F.A new colorimetric method for the determination of alkaline phosphatase with indoxyl phosphateAnal. Biochem.11(1)54-64(1965) 2.Fanjul-Bolado, P., GonzÁlez-GarcÍa, M.B., and Costa-GarcÍa, A.Detection of leucoindigo in alkaline phosphatase and peroxidase based assays using 3-indoxyl phosphate as substrateAnal. Chim. Acta534(2)231-238(2005)